Anti-SARS-CoV-2 NSP12 Rabbit Polyclonal Antibody for WB, ELISA - P0DTD1

Core Product Specifications and Parameters

Biological Background: SARS-CoV-2 NSP12 Function and Localization

• NSP12 is the RNA-directed RNA polymerase (RdRp) that catalyzes the transcription of viral genomic and subgenomic RNAs, essential for SARS-CoV-2 replication. Related references: PMID:32277040 PMID:32358203 PMID:32438371
• Together with the accessory proteins nsp7 and nsp8, NSP12 forms a highly processive replication complex required for efficient RNA synthesis. Related references: PMID:32277040 PMID:32358203 PMID:32438371
• The kinase-like NiRAN domain of NSP12 attaches one or more nucleotides to the N-terminus of NSP9, creating a covalent RNA-protein primer for transcription initiation. Related references: PMID:37794589
• NSP12 executes discontinuous transcription by pausing at transcription-regulating sequences (TRS) and jumping to the leader TRS, leading to the production of subgenomic mRNAs. Related references: PMID:35706445
• NSP12 is a subunit of the viral RNA capping enzyme and catalyzes the guanylyltransferase reaction forming the core cap structure GpppA-RNA, crucial for mRNA stability and immune evasion. Related references: PMID:35944563
• Subcellularly, NSP12 localizes predominantly to the host cytoplasm, and is recruited to double-membrane vesicles and perinuclear replication complexes derived from the endoplasmic reticulum.
• As a central player in coronavirus replication, NSP12 is the target of major antiviral drugs such as remdesivir and molnupiravir.

Experimental Guidance and Technical Tips

• For Western blotting, be aware that the antibody may detect both the full replicase polyprotein precursor (~794 kDa) and the mature NSP12 after proteolytic processing. It is advisable to validate detection in SARS-CoV-2-infected cell lysates or recombinant NSP12-expressing systems.
• In ELISA applications, the polyclonal nature can enhance sensitivity; however, cross-reactivity with unrelated proteins or other coronaviruses should be ruled out using proper controls.
• As the immunogen is a synthetic peptide (amino acids 200–300 of NSP12), confirm that the epitope remains accessible under your assay conditions (denatured vs. native).
• For intracellular detection by immunofluorescence, consider that NSP12 is associated with viral replication complexes on host membranes; fixation/permeabilization may need optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD antibodies and ancillary reagents, supporting virus research and assay development from concept to clinic. This SARS-CoV-2 NSP12 polyclonal antibody is suited for virology applications including western blot and ELISA; we can also supply validated antibody pairs, recombinant antigens, and bulk consumables. For custom development needs or rare target sourcing, our team offers expert consulting and tailored supply solutions to accelerate your project.