Anti-SARS-CoV-2 NSP13 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P0DTD1

Core Product Specifications and Parameters

Biological Background: SARS-CoV-2 NSP13 Function and Localization

• SARS-CoV-2 NSP13 is the viral helicase, a component of the replicase polyprotein 1ab (P0DTD1), essential for viral RNA synthesis.
• Exhibits RNA and DNA duplex-unwinding activities with 5' to 3' polarity, dependent on magnesium. Related references: PMID:33232691
• Binds to host TBK1 and inhibits TBK1 phosphorylation, decreasing IRF3 phosphorylation and reducing IFN-beta production, contributing to innate immune evasion. Related references: PMID:32979938
• Contains an N-terminal zinc-binding domain and is classified under keywords including Helicase, ATP-binding, Zinc-finger, and Viral immunoevasion.
• Primarily localizes to the host cytoplasm, associating with viral replication-transcription complexes.
• Plays a critical role in viral replication and transcription, making it a potential target for antiviral therapeutic development.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length NSP13 (amino acids 1-601). While the antibody may detect the uncleaved replicase polyprotein (~794 kDa), mature NSP13 is ~67 kDa. For WB, consider using gradient gels to cover both size ranges if needed.
• For IF/ICC, optimize fixation and permeabilization protocols for viral protein detection in infected cells.
• In ELISA, this antibody can be used for direct or sandwich detection of recombinant or native NSP13 from SARS-CoV-2 preparations.
• Validate antibody specificity using appropriate controls, such as uninfected cell lysates or knockout/knockdown systems where applicable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering reliable access to antibodies like this SARS-CoV-2 NSP13 polyclonal antibody for viral research and diagnostic development. From validated antibody pairs and bulk ancillary reagents to rare-target raw-material sourcing, we support your workflow from concept to clinic. Connect with us to streamline your procurement and accelerate your COVID-19 research projects.