Anti-SARS-CoV-2 NSP16 Rabbit Polyclonal Antibody for WB, ELISA - P0DTD1

Core Product Specifications and Parameters

Biological Background: NSP16 Function and Localization

• NSP16 is the 2'-O-methyltransferase domain of the SARS-CoV-2 replicase polyprotein 1ab, responsible for methylating the 5' cap structure of viral mRNAs. Related references: PMID:35944563
• The methylation activity is crucial for immune evasion, allowing viral mRNAs to avoid recognition by host restriction factors IFIT1 and IFIT3. Related references: PMID:35944563 PMID:36722972 PMID:36285486
• NSP16 requires an N7-methyl guanosine cap on the mRNA substrate for binding and enzymatic activity. Related references: PMID:35944563
• It may also interfere with host mRNA splicing by interacting with pre-mRNA recognition domains of U1 and U2 snRNAs in the nucleus. Related references: PMID:33080218
• Subcellular localization includes the host cytoplasm, perinuclear region, and nucleus, consistent with its roles in viral replication and host manipulation.
• As part of the viral replication-transcription complex, NSP16 is essential for the production of mature, translation-competent viral mRNAs.
• The polyprotein is processed by viral proteases into 16 non-structural proteins; NSP16 constitutes the C-terminal domain.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 236–290 of NSP16, a region that may be exposed in denaturing conditions, supporting its use in Western blot.
• Validate the antibody in SARS-CoV-2 infected cell lysates or recombinant NSP16 protein to confirm specificity.
• For ELISA, consider pairing with a suitable capture or detection antibody; perform titration to optimize signal-to-noise ratios.
• Since the predicted molecular weight of NSP16 is approximately 33 kDa, confirm the observed band size under experimental conditions, keeping in mind possible post-translational modifications or processing intermediates.
• Include appropriate positive and negative controls to distinguish specific signal from background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this rabbit polyclonal antibody against SARS-CoV-2 NSP16 as part of our comprehensive IVD raw-material portfolio, supporting diagnostic and research efforts into coronavirus biology. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—streamlining your development from concept to clinic. Contact us to explore tailored supply solutions and technical consultation for your next project.