Phospho-EPHA2-S897 Rabbit pAb for WB, ELISA - P29317

Core Product Specifications and Parameters

Biological Background: Ephrin type-A receptor 2 Function and Localization

• EPHA2 is a receptor tyrosine kinase that binds ephrin-A ligands, mediating contact-dependent bidirectional signaling (forward and reverse). This regulates cell migration, integrin-mediated adhesion, proliferation, and differentiation.
• It plays roles in development, angiogenesis, hindbrain morphogenesis, and mammary gland epithelial branching.
• EPHA2 acts as a receptor for hepatitis C virus (HCV) and human cytomegalovirus (HCMV), facilitating viral entry into host cells.
• Subcellular localization includes the cell membrane, ruffle membrane, lamellipodium membrane, and focal adhesions, supporting its role in cell motility and adhesion.
• The protein is highly expressed in tissues rich in epithelial cells, such as skin, intestine, lung, and ovary, as well as in brain and glioma tissues.
• Post-translational modifications include phosphorylation at serine 897 (the target of this antibody), which is critical for signaling. It also undergoes ubiquitination and glycosylation.

Experimental Guidance and Technical Tips

• This phospho-specific antibody was raised against a synthetic peptide phosphorylated at serine 897; consider verifying specificity by comparing signals in phosphatase-treated versus untreated samples.
• For Western blotting, EPHA2 has a predicted molecular weight of ~108 kDa; confirm the band size and use appropriate positive controls (e.g., pervanadate-treated cells to enhance phosphorylation).
• In ELISA applications, include both phosphorylated and non-phosphorylated peptide coatings to assess phospho-specificity.
• Given the reported expression in epithelial tissues and certain cancers, relevant sample types may include lysates from skin, lung, ovary, or glioma cell lines.
• Sequence alignment of the phospho-site across human, mouse, and rat shows conservation, supporting the observed cross-reactivity; validate in your specific model system.

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CamelBio is your trusted one-stop partner for IVD raw-material sourcing, offering specialized antibodies such as this phospho-EPHA2-S897 tool to support your assay development. With a comprehensive portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, CamelBio ensures consistent quality and supply reliability. Contact us to accelerate your projects in cancer, developmental biology, and virology research.