Phospho-EGFR-Y869 Rabbit Polyclonal Antibody for WB and ELISA - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• EGFR is a receptor tyrosine kinase that binds EGF family ligands (including EGF, TGF-alpha, AREG, epigen, betacellulin, epiregulin, and HBEGF), leading to receptor dimerization and autophosphorylation. Related references: PMID:10805725 PMID:27153536 PMID:2790960 PMID:12297049 PMID:15611079 PMID:17909029

• Ligand-activated EGFR triggers multiple intracellular signaling cascades, including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC, and STAT modules, as well as NF-kappa-B signaling. Related references: PMID:27153536 PMID:11116146

• EGFR directly phosphorylates other proteins such as RGS16 (enhancing its GTPase activity) and MUC1 (promoting its interaction with SRC and beta-catenin), linking to G protein and cell adhesion pathways. Related references: PMID:11602604 PMID:11483589

• EGFR positively regulates cell migration by interacting with CCDC88A/GIV, which retains EGFR at the cell membrane and sustains signaling. Related references: PMID:20462955

• The EGFR gene (also known as ERBB1, HER1) encodes a receptor tyrosine kinase that belongs to the ERBB family; an alternatively spliced isoform (isoform 2) may act as an antagonist of EGF action and is expressed in ovarian cancers.

• EGFR is predominantly localized at the cell membrane but is also detected in the endoplasmic reticulum, Golgi apparatus, nucleus membrane, endosomes, and can be secreted.

• EGFR is a phosphoprotein with key tyrosine phosphorylation sites (including Y869) that regulate its kinase activity, and undergoes glycosylation, ubiquitination, and other post-translational modifications.

• Ubiquitously expressed in human tissues; EGFR acts as a host cell receptor for hepatitis C virus (HCV), facilitating viral entry into hepatocytes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to residues around Y869 of human EGFR. To confirm phospho-specificity, include phosphatase-treated controls or compete with non-phosphorylated peptide.
• For western blot, use EGF-stimulated cell lysates and expect a band at ~134 kDa; cross-reactivity with mouse and rat EGFR is reported, but validate in your species of interest.
• For ELISA, consider coating with a phosphorylated peptide and optimizing blocking and detection conditions to enhance signal-to-noise ratio.
• Avoid harsh stripping conditions when reprobing blots, as this may remove the phospho-specific signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated phospho-specific antibodies, recombinant proteins, and ancillary reagents. For EGFR signaling research and diagnostic assay development, we provide reliable, high-quality antibodies and can support sourcing for rare or custom targets. From initial concept to clinical application, CamelBio streamlines your supply chain as a trusted partner.