Phospho-RPA2-S4/S8 Rabbit pAb - P15927

Core Product Specifications and Parameters

Biological Background: Replication Protein A 32 kDa Subunit Function and Localization

• The Replication protein A 32 kDa subunit (RPA2, also known as RPA32, RP-A p32, RP-A p34) is a component of the heterotrimeric replication protein A (RPA) complex, which includes RPA1, RPA2, and RPA3.
• RPA binds and stabilizes single-stranded DNA (ssDNA) intermediates generated during DNA replication and DNA stress, preventing reannealing and facilitating the recruitment of DNA metabolism proteins.
• In the cellular response to DNA damage, RPA is crucial for DNA repair and checkpoint activation. It recruits the ATR-ATRIP kinase complex to DNA damage sites, initiating the DNA damage response cascade.
• RPA recruits key repair factors to chromatin, including RAD51 and RAD52 for double-strand break repair, as well as XPA and XPG for nucleotide excision repair (NER). It also participates in base excision repair (BER) via interaction with UNG.
• RPA stimulates the 5'-3' helicase activity of BRIP1/FANCJ, which is involved in interstrand crosslink repair.
  Related references:
  PMID:17596542
• RPA primarily localizes to the nucleus and is found in PML bodies, consistent with its roles in DNA metabolism.
• RPA2 is a phosphoprotein subject to various post-translational modifications, including acetylation, ubiquitylation, and alternative splicing, which regulate its function in DNA damage signaling.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around S4 and S8 of human RPA2. For Western blot confirmation, consider using phosphatase-treated cell lysates to verify phosphorylation-specific signal loss.
• The predicted molecular weight of unmodified RPA2 is 29 kDa; however, phosphorylation or other modifications may affect migration. Use appropriate molecular weight markers and validate in your sample system.
• This antibody is recommended for WB and ELISA. For ELISA, titrate the antibody to determine optimal working dilution, and include a non-phosphorylated peptide control to ensure specificity.
• Cross-reactivity with rat RPA2 has been reported; if using rat samples, validate performance under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers, labs, and research institutes with comprehensive access to validated antibodies, antigen pairs, and ancillary reagents. With expertise in DNA replication and damage response targets like RPA2, we offer bulk quantities, customization, and technical support to accelerate your assay development from concept to clinic.