Anti-Phospho-KRAS-S89 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P01116

Core Product Specifications and Parameters

Biological Background: KRAS (GTPase KRas) Function and Localization

• KRAS (GTPase KRas), also known as K-Ras 2, Ki-Ras, c-K-ras, is encoded by the KRAS gene (also known as KRAS2, RASK2) and belongs to the Ras family of small GTPases.
• Serves as a signal transducer in the Ras-MAPK signaling pathway, regulating cell proliferation and survival. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Related references: PMID:22711838 PMID:23698361 PMID:20949621 PMID:39809765
• Activates MAPK1/MAPK3, resulting in phosphorylation and subsequent degradation of GJA1 (By similarity).
• In colorectal cancer (CRC) cells, promotes oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in a ZNF304-dependent manner. Related references: PMID:24623306
• Recognized by LZTR1, which mediates its ubiquitination by a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex. Related references: PMID:40934300
• Subcellular localization includes the cell membrane, endomembrane system, and cytoplasm.
• Tissue specificity: expressed throughout the epidermis, but weakly in the stratum corneum and dermis (at protein level).
• Post-translational modifications encompass acetylation, ubiquitination, prenylation, palmitoylation, and methylation, which regulate membrane association and activity.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphorylated peptide around serine 89 of human KRAS; it is designed to specifically recognize KRAS only when phosphorylated at S89. To confirm phospho-specificity, consider treating samples with lambda phosphatase and observe loss of signal in Western blot.
• Recommended applications include Western blot (WB), immunohistochemistry (IHC-P), and ELISA. For WB, human, mouse, and rat samples are expected to show a band at approximately 22 kDa. Validate the antibody in your specific experimental system by including positive and negative controls.
• For IHC-P, optimize antigen retrieval and antibody dilution; phospho-specific antibodies may require careful handling to preserve phosphorylation status.
• Due to the cross-reactivity across human, mouse, and rat, this antibody is suitable for translational studies; however, always verify reactivity in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop sourcing for IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies. This phospho-KRAS-S89 antibody supports cancer signaling research and oncogene studies, and is part of CamelBio's comprehensive portfolio of phosphorylated antibodies and ancillary reagents for assay development.