Anti-Phospho-RB1 (Ser807/Ser811) Rabbit Polyclonal Antibody for WB, ELISA - P06400

Core Product Specifications and Parameters

Biological Background: Retinoblastoma-associated protein Function and Localization

• Tumor suppressor that regulates G1/S transition: hypophosphorylated RB1 binds E2F transcription factors, preventing transcription of E2F-responsive genes; cyclin/CDK-dependent phosphorylation (including at Ser807/Ser811) induces dissociation from E2Fs, activating transcription. Related references: PMID:10499802
• Promotes G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C. Related references: PMID:15084261
• Directly involved in heterochromatin formation, maintaining overall chromatin structure and stabilizing histone methylation; recruits histone methyltransferases SUV39H1, KMT5B, and KMT5C, leading to epigenetic transcriptional repression, including control of histone H4 'Lys-20' trimethylation.
• Mediates transcriptional repression by SMARCA4/BRG1 through recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter; in resting neurons, calcium influx activates calcineurin to dephosphorylate RB1, releasing the repressor complex (By similarity).
• During viral infection, interactions with SV40 large T antigen, HPV E7 protein, or adenovirus E1A protein disrupt the RB1-E2F1 complex, inactivating tumor suppressor function.
• Subcellular location: Nucleus, Cytoplasm.
• Expressed in retina and foreskin keratinocytes (at protein level).
• Keywords: Tumor suppressor, Cell cycle, Chromatin regulator, Phosphoprotein, Host-virus interaction, Repressor.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to residues around Ser807 and Ser811 of human RB1; consider verifying specificity by treating cell lysates with lambda phosphatase to confirm phosphorylation-dependent detection.
• This antibody is suitable for Western blot (WB) and ELISA; for WB, a band around 106 kDa is expected in phosphorylated RB1 samples.
• When performing ELISA, the phosphopeptide immunogen may serve as a positive control; titrate antibody concentration to optimize signal-to-noise ratio.
• For species cross-reactivity, this antibody recognizes human, mouse, and rat RB1; validate in the relevant sample system prior to critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to a comprehensive portfolio of IVD raw materials, including phosphorylation-specific antibodies like this anti-phospho-RB1 (Ser807/Ser811) antibody. Whether you require validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or rare target raw-material sourcing for cell cycle and oncology research, CamelBio supports your projects from concept to clinic. Contact us for technical consultation and streamlined procurement solutions.