Anti-Phospho-RB-S807/811 Rabbit Monoclonal Antibody for WB - P06400

Core Product Specifications and Parameters

Biological Background: Retinoblastoma-associated protein (RB1) Function and Localization

• Tumor suppressor protein that regulates the G1/S transition: hypophosphorylated RB1 binds and inhibits E2F transcription factors, repressing cell cycle progression genes. Cyclin/CDK-mediated phosphorylation releases E2Fs to activate S-phase entry (PubMed:10499802). Related references: PMID:10499802
• Also promotes the G0-G1 transition through phosphorylation by CDK3/cyclin-C (PubMed:15084261). Related references: PMID:15084261
• Directly involved in heterochromatin formation by maintaining chromatin structure and stabilizing histone methylation; recruits histone methyltransferases SUV39H1, KMT5B and KMT5C to control H4K20 trimethylation.
• Subcellular localization: Found predominantly in the nucleus, but can shuttle to the cytoplasm.
• Tissue expression: Detected in retina and foreskin keratinocytes (at protein level) (PubMed:20940255). Related references: PMID:20940255
• RB1 is a phosphoprotein with key regulatory phosphorylation sites, including S807 and S811, which modulate its interaction with E2Fs.
• Microbial interactions: Viral oncoproteins (SV40 large T antigen, HPV E7, adenovirus E1A) bind and inactivate RB1, disrupting its tumor suppressor function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around human RB1 S807/S811; the antibody is expected to specifically detect RB1 phosphorylated at these sites.
• Recommended applications: WB (expected band ~106 kDa), IP, IF/ICC, IF-P, and ELISA. Optimize antibody dilutions for each assay.
• To preserve phosphorylation signals, include phosphatase inhibitors in lysis and wash buffers during sample preparation.
• Cross-reactivity with mouse is indicated; validate in your experimental model if using mouse samples.
• Use appropriate positive and negative controls (e.g., phosphorylated vs. dephosphorylated lysates) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing partner for IVD raw materials, offering this high-quality anti-phospho-RB1 antibody for cell cycle and cancer research. Our portfolio includes validated antibody pairs, recombinant proteins, and ancillary reagents to support your diagnostic assay development from concept to clinic.