Anti-Phospho-MET-Y1234/1235 Rabbit pAb for WB, ELISA - P08581

Core Product Specifications and Parameters

Biological Background: MET Function and Localization

• MET, also known as Hepatocyte growth factor receptor (HGFR), proto-oncogene c-Met, and Scatter factor receptor, is a receptor tyrosine kinase that transduces signals from the extracellular matrix upon binding to its ligand HGF. It regulates key processes such as proliferation, scattering, morphogenesis, and survival.
• Upon ligand binding, MET undergoes autophosphorylation at tyrosine residues including Y1234 and Y1235 in the activation loop, which creates docking sites for downstream effectors like PI3K, PLCG1, SRC, GRB2, STAT3, and GAB1. This triggers multiple cascades: RAS-ERK (morphogenetic effects), PI3K-AKT (prosurvival), and PLCgamma-PKC pathways.
• During embryonic development, MET signaling is essential for gastrulation, neuronal precursor migration, angiogenesis, kidney formation, and skeletal muscle development (muscle progenitor migration and secondary myoblast proliferation). In adults, it contributes to wound healing, organ regeneration, tissue remodeling, and hematopoiesis.
• MET is primarily localized to the cell membrane, but a secreted form also exists. It is expressed in normal hepatocytes and epithelial cells lining the stomach, small and large intestines, as well as in basal keratinocytes of esophagus and skin. High expression is observed in liver, gastrointestinal tract, thyroid, kidney, and brain, with detectable protein levels in metaphyseal bone. Related references: PMID:26637977
• The protein acts as a receptor for internalin InlB from Listeria monocytogenes, mediating bacterial entry into host cells.
• MET is classified as a tyrosine-protein kinase, glycoprotein, and phosphoprotein, and is associated with chromosomal rearrangements linked to disease. It is also subject to alternative splicing and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic phosphorylated peptide spanning Y1234 and Y1235 of human MET. For WB and ELISA, consider including non-phosphopeptide competition or phosphatase-treated lysates to confirm phosphorylation specificity.
• For WB, a band at approximately 156 kDa is expected. Since MET is a membrane protein, use appropriate lysis buffers (e.g., RIPA) and include protease/phosphatase inhibitors. For ELISA, validate the antibody with coated phospho-peptide and appropriate controls.
• Stimulating cells with HGF before lysis can enhance detection of phosphorylated MET at these sites. Be aware of basal phosphorylation levels in your cell line of interest.
• This antibody is validated for human samples; cross-reactivity with other species is not established.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including phosphorylated antibodies like this anti-Phospho-MET-Y1234/1235 rabbit pAb, to support research into HGF/MET signaling, cancer biology, and therapeutic development. As a reliable sourcing partner, we provide validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents to streamline your assay development. Contact us to discuss customized sourcing solutions for rare targets and phospho-specific detection needs.