Anti-Phospho-eIF4EBP1 (T70) Rabbit Monoclonal Antibody for WB, ELISA - Q13541

Core Product Specifications and Parameters

Biological Background: eIF4EBP1 Function and Localization

• eIF4EBP1 (eukaryotic translation initiation factor 4E-binding protein 1) is also known as PHAS-I, BP-1, or 4E-BP1.
• It functions as a translational repressor by binding to eIF4E and preventing its assembly into the eIF4F complex; this inhibition is relieved upon hyperphosphorylation, which dissociates the complex.
• The phosphorylation status of eIF4EBP1 is regulated by growth factors and hormones through the MAP kinase and mTORC1 signaling pathways, thereby controlling cap-dependent translation.
• eIF4EBP1 localizes to both the cytoplasm and nucleus, consistent with its role in translation and potentially other nuclear functions.
• Key post-translational modifications include phosphorylation (multiple sites, including Thr70), acetylation, and ubiquitination, which modulate its activity and stability.
• As a phosphoprotein and translation regulator, eIF4EBP1 is implicated in cellular growth, proliferation, and metabolism.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phospho-peptide corresponding to Thr70 of human eIF4EBP1. For best results, consider verifying specificity on phosphorylated versus non-phosphorylated samples in your experimental system.
• The recommended applications are Western blot (WB) and ELISA; if using for other applications, validate under your own conditions.
• Cross-reactivity has been observed with human and mouse eIF4EBP1; sequence alignment can guide the likelihood of reactivity with other species.
• For WB, consider using fresh cell lysates and phosphatase inhibitors to preserve phosphorylation state.
• The observed protein weight is ~13 kDa; adjust gel conditions accordingly for optimal resolution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, providing validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. For researchers investigating mTOR signaling and translation control, CamelBio can supply high-quality phospho-specific and total antibodies to support assay development and routine detection.