Anti-EFNA3 Rabbit Polyclonal Antibody for WB, ELISA - P52797

Core Product Specifications and Parameters

Biological Background: EFNA3 (Ephrin-A3) Function and Localization

• Ephrin-A3 (EFNA3), also known as EFL-2, EHK1-L, or LERK-3, is a member of the ephrin family and exists in multiple isoforms generated by alternative splicing.
• It functions as a GPI-anchored cell-surface ligand for Eph receptors, mediating contact-dependent bidirectional signaling (forward and reverse) essential for neuronal, vascular, and epithelial development.
• Ephrin-A3 binds promiscuously to Eph receptors on adjacent cells, regulating processes such as cell migration, repulsion, and adhesion.
• The protein is localized to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor, with the mature polypeptide oriented extracellularly.
• Post-translational modifications include glycosylation and disulfide bond formation, and the protein features a signal peptide for secretion.
• EFNA3 is expressed in a wide range of tissues, including brain, skeletal muscle, spleen, thymus, prostate, testis, ovary, small intestine, and peripheral blood leukocytes.

Experimental Guidance and Technical Tips

• For Western blot, consider using lysates from tissues with known EFNA3 expression such as brain or skeletal muscle; validate with appropriate positive and negative controls.
• The antibody’s mouse cross-reactivity makes it a candidate for studies in mouse models; however, confirm reactivity and specificity in your model system.
• Given the GPI anchor, ensure that sample preparation methods do not compromise epitope accessibility; consider using detergent-based lysis buffers.
• For ELISA, polyclonal antibodies may provide broader epitope recognition; assess cross-reactivity with other ephrin family members if necessary.
• The immunogen spans the extracellular domain (aa 23-214), which contains the receptor-binding region; the antibody may compete with ligand-receptor interactions in functional assays.

CamelBio: Your One-Stop Sourcing Bridge

This EFNA3 antibody, targeting a key developmental signaling ligand, exemplifies the high-quality IVD reagents we supply. CamelBio serves as a one-stop sourcing partner for diagnostic manufacturers, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement to streamline your assay development from concept to clinic.