Anti-EPHA3 Rabbit Polyclonal Antibody for WB, ELISA - P29320

Core Product Specifications and Parameters

Biological Background: EPHA3 Function and Localization

• EPHA3 (Ephrin type-A receptor 3) is a receptor tyrosine kinase, also known as EK4, HEK, TYRO4, and ETK1, encoded by the EPHA3 gene on human chromosome 3. It belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family.
• EPHA3 mediates contact-dependent bidirectional signaling: forward signaling through the receptor upon binding ephrin-A ligands (preferentially EFNA5) and reverse signaling through the ephrin ligand.
  Related references: PMID:11870224 PMID:12794130
• Upon activation by EFNA5, EPHA3 regulates cell-cell adhesion, cytoskeletal organization, and cell migration (PubMed:11870224). Activation by EFNA1 inhibits epithelial-to-mesenchymal transition in cardiac cells and contributes to heart development (PubMed:12794130).
  Related references: PMID:11870224 PMID:12794130
• EPHA3 is involved in retinotectal mapping of neurons and may control the segregation (but not guidance) of motor and sensory axons during neuromuscular circuit development.
• Subcellularly, EPHA3 is localized to the cell membrane, and a secreted isoform has also been reported.
• EPHA3 is widely expressed in human tissues, with the highest levels observed in the placenta.
• Key post-translational modifications include phosphorylation on tyrosine residues (phosphoprotein), and it is a glycoprotein. It contains an ATP-binding site and a kinase domain, characteristic of tyrosine-protein kinases.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the extracellular domain of EPHA3, suggesting the antibody may recognize the extracellular portion of the receptor.
• For Western blotting (WB), consider using whole-cell lysates from cell lines expressing EPHA3, such as placental or cardiac tissues, given its tissue distribution.
• Due to cross-reactivity with mouse and rat EPHA3, the antibody may be suitable for studies in these species; however, validation in the relevant sample system is recommended.
• For ELISA, optimize coating antigen and detection conditions, and validate specificity using recombinant EPHA3 protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials. For studies of ephrin receptor signaling, cell adhesion, and developmental biology, we offer validated anti-EPHA3 antibodies and can assist in sourcing complementary reagents such as recombinant proteins and detection antibodies. Our end-to-end support covers everything from antibody pair development to bulk reagent supply, efficiently bridging your project from concept to clinic.