Phospho-EPHA2-Y594 Rabbit pAb for WB and ELISA - P29317

Core Product Specifications and Parameters

Biological Background: EPHA2 Function and Localization

• EPHA2 encodes the ephrin type-A receptor 2, a receptor tyrosine kinase that transduces signals upon binding to membrane-bound ephrin-A ligands on adjacent cells, initiating bidirectional signaling (forward through the receptor and reverse through the ligand).
• It regulates a variety of cellular processes including cell migration, integrin-mediated adhesion, proliferation, and differentiation, partly through modulation of the ERK1/ERK2 signaling pathway.
• EPHA2 participates in developmental processes such as angiogenesis, hindbrain patterning, and branching morphogenesis in the mammary gland, and contributes to lens fiber cell organization and transparency.
• Beyond normal physiology, EPHA2 acts as a receptor for hepatitis C virus (HCV) and human cytomegalovirus (HCMV), mediating viral entry into host cells.
• The protein is localized at the cell membrane with enrichment in dynamic cell protrusions including ruffle membranes, lamellipodia, and focal adhesions, consistent with its roles in cell adhesion and motility.
• EPHA2 is highly expressed in epithelial-rich tissues such as skin, intestine, lung, and ovary, and is also detected in brain and glioma cell lines.
• Key structural features include an extracellular ephrin-binding domain, a single-pass transmembrane domain, and an intracellular kinase domain with the phosphorylatable tyrosine residue Y594.
• EPHA2 is classified under multiple functional keywords: tyrosine-protein kinase, ATP-binding, receptor, glycoprotein, alternative splicing, and angiogenesis, underscoring its central role in signal transduction and disease.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide encompassing Y594, which should provide high specificity for the phosphorylated form—confirm specificity by treating samples with λ-phosphatase before western blot.
• For western blotting, include phosphatase inhibitors during lysis and consider using positive controls such as ephrin-A1-stimulated cells or epithelial cancer cell lines known to overexpress EPHA2.
• Due to reported cross-reactivity with rat, this antibody may be suitable for studies in rat models; however, validate the reactivity and optimal dilution in your specific sample system.
• In ELISA, use a direct or indirect format with phospho-EPHA2 capture antigen; assay conditions should be empirically optimized for coating concentration and detection antibody dilution.
• Note the predicted molecular weight of EPHA2 is approximately 108 kDa—always run appropriate molecular weight markers and verify band size.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes streamlined access to a broad portfolio of IVD raw materials, including this Phospho-EPHA2-Y594 rabbit polyclonal antibody. We support your receptor tyrosine kinase and viral entry research programs with validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary reagents, and specialized sourcing for rare-target raw materials. Partner with us to accelerate your development from concept to clinical application.