Phospho-E-Cadherin-S838/S840 Rabbit pAb - P12830

Core Product Specifications and Parameters

Biological Background: E-cadherin Function and Localization

• E-cadherin forms calcium-dependent homophilic adhesions, essential for sorting heterogeneous cell types and maintaining epithelial barrier function.
  Related references: PMID:11976333

• It regulates epithelial cell mobility and proliferation, and contributes to radial actin fiber organization via interaction with AMOTL2.

• E-cadherin facilitates early desmosome junction formation by recruiting DSG2 and DSP to desmosomal plaques.
  Related references: PMID:29999492

• The protein is a known invasive suppressor and serves as a ligand for integrin alpha-E/beta-7.

• Cleavage product E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors, inhibiting APP C99 and C83 production.

• Subcellularly, E-cadherin localizes to adherens junctions, desmosomes, cell membrane, endosomes, Golgi, and can be secreted; tissue expression includes granuloma macrophages, skin, esophageal epithelium, and liver.

• Post-translational modifications include phosphorylation, disulfide bond formation, glycosylation, and cleavage; E-cadherin is a phosphoprotein with calcium and metal-binding properties.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to residues around S838/S840 of human E-cadherin; specificity for the phosphorylated form should be validated by phosphatase treatment or comparison with non-phosphorylated controls in WB.
• For Western blotting, E-cadherin migrates at approximately 97 kDa; consider using 8-10% SDS-PAGE gels and appropriate transfer conditions.
• While validated for WB and ELISA, optimization of antibody dilution and incubation conditions is recommended for each experimental system.
• Phosphorylation at S838/S840 may be regulated by specific kinases; review the literature to identify relevant stimuli and inhibitors in your cell model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, connecting researchers and diagnostic manufacturers with high-quality reagents for cell adhesion and cancer biology studies. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized sourcing for rare or specialized targets. With expertise spanning from concept to clinic, we support your development of robust immunoassays and diagnostic tools.