Phospho-EGFR-Y845 Rabbit pAb for WB and ELISA - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• EGFR is a receptor tyrosine kinase that binds EGF family ligands, leading to receptor dimerization and autophosphorylation on tyrosine residues. Related references: PMID:10805725, PMID:27153536, PMID:2790960, PMID:12297049, PMID:15611079, PMID:17909029
• Phosphorylated EGFR activates multiple downstream signaling cascades, including the RAS-RAF-MEK-ERK and PI3K-AKT pathways, and can directly phosphorylate substrates like RGS16 and MUC1. Related references: PMID:27153536, PMID:11116146, PMID:11602604, PMID:11483589
• Isoform 2 of EGFR may act as an antagonist of EGF action.
• EGFR enhances cell migration via interaction with CCDC88A/GIV and is involved in learning, memory, and pain signaling. Related references: PMID:20462955
• As a host factor, EGFR serves as a receptor for hepatitis C virus (HCV) entry into hepatocytes.
• EGFR localizes to the cell membrane, endosomes, Golgi apparatus, endoplasmic reticulum, nucleus, and can be secreted.
• EGFR is ubiquitously expressed, with isoform 2 notably expressed in ovarian cancers.
• EGFR is classified as a phosphoprotein, proto-oncogene, and kinase, with functional roles in development, signal transduction, and host-virus interactions.

Experimental Guidance and Technical Tips

• Since this antibody targets a phosphorylation site, include phosphatase inhibitor cocktails during cell lysis to preserve the phospho-epitope.
• For Western blot, use EGF-stimulated cell lysates as a positive control to confirm target phosphorylation and antibody reactivity.
• In ELISA assays, include both phosphorylated and non-phosphorylated peptide controls to assess binding specificity.
• As a rabbit polyclonal antibody, validate lot-to-lot consistency with reference standards before critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a full range of IVD raw materials, including validated antibodies, antigens, and detection reagents for EGFR signaling research. Our one-stop sourcing platform supports diagnostic manufacturers and laboratories with bulk ancillary reagents, custom procurement, and technical consulting to streamline assay development. Explore our EGFR antibody portfolio for consistent performance and dedicated technical support tailored to your workflow.