Anti-EPHA2 Rabbit Monoclonal Antibody for WB and ELISA - P29317

Core Product Specifications and Parameters

Biological Background: EPHA2 Function and Localization

• Ephrin type-A receptor 2 (EPHA2) is a receptor tyrosine kinase that binds ephrin-A ligands, triggering bidirectional signaling pathways that regulate cell migration, adhesion, proliferation, and differentiation.
• EPHA2 mediates contact-dependent communication through forward signaling (downstream of the receptor) and reverse signaling (downstream of the ephrin ligand), crucial for cellular responses.
• Subcellularly, EPHA2 is primarily located at the cell membrane, enriched at ruffle membranes, lamellipodia, and focal adhesions, supporting its role in cell motility and adhesion.
• EPHA2 is highly expressed in epithelial cell-rich tissues such as skin, intestine, lung, and ovary, and is also detected in brain and glioma tissues.
• Beyond developmental functions in angiogenesis and tissue morphogenesis, EPHA2 serves as an entry receptor for hepatitis C virus (HCV) and human cytomegalovirus (HCMV), facilitating viral infection.
• Post-translational modifications, including glycosylation and phosphorylation, modulate EPHA2 signaling activity and protein interactions.

Experimental Guidance and Technical Tips

• Given that the immunogen spans the extracellular domain (amino acids 25-534), this antibody likely recognizes the extracellular region of EPHA2, making it suitable for detecting both full-length and soluble forms.
• For Western blot (WB), standard reducing SDS-PAGE is recommended; expect a band at approximately 108 kDa. Optimize lysate preparation and primary antibody dilution for your specific sample.
• For ELISA applications, consider direct coating of the recombinant protein or use as a capture antibody; validate with your target analyte in the appropriate matrix.
• As a recombinant monoclonal antibody, it offers high lot-to-lot reproducibility, supporting consistent results in long-term studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between diagnostic developers and premium IVD raw materials, providing a single point of access for research-grade antibodies and related reagents. This EPHA2 rabbit monoclonal antibody is part of our expanding portfolio of monoclonal antibodies validated for WB and ELISA, ideal for cell adhesion, migration, and viral entry studies. We also offer bulk sourcing options, customized antibody development, and ancillary reagents to support your assay development from concept to clinic.