Anti-Titin (TTN) Rabbit Polyclonal Antibody for IF-P and ELISA - Q8WZ42

Core Product Specifications and Parameters

Biological Background: Titin Function and Localization

• Titin, also known as connectin, is a giant protein that serves as a key component in the assembly and functioning of vertebrate striated muscles, providing elastic connections within the sarcomere.
• By linking individual microfilaments, titin balances forces between the two halves of the sarcomere, and its size and extensibility determine sarcomere extensibility and muscle passive elasticity.
• In non-muscle cells, titin participates in chromosome condensation and segregation during mitosis, and may connect the nuclear lamina to chromatin or nuclear actin during interphase.
• Titin is expressed in multiple isoforms: cardiac muscle expresses isoforms 3, 7, and 8; skeletal muscle expresses isoform 4; and isoform 6 is found in skeletal muscle at the protein level.
• It localizes to both the cytoplasm and nucleus, consistent with its dual roles in muscle structure and chromosome dynamics.
• The protein features immunoglobulin-like domains, a serine/threonine kinase domain, and binding sites for ATP, calcium, and calmodulin, highlighting its complex regulatory functions.
• Mutations in TTN are associated with a spectrum of human diseases, including cardiomyopathy and skeletal myopathies such as limb-girdle muscular dystrophy and hereditary myopathy with early respiratory failure (HMERF).

Experimental Guidance and Technical Tips

• For immunofluorescence on paraffin sections (IF-P), optimize antigen retrieval and antibody dilution, as staining intensity may vary with fixation and embedding conditions.
• To validate ELISA performance, consider using the recombinant immunogen fragment (aa 5347-5604) or full-length titin as a coating antigen, and include appropriate controls.
• When applying this antibody in mouse or rat tissues, confirm reactivity by comparing signal with human positive controls, given potential sequence differences in the immunogen region across species.
• Due to the exceptionally large molecular weight of titin (~3816 kDa), standard electrophoresis and blotting may require specialized protocols; the antibody has been validated only for IF-P and ELISA, not western blotting.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement of high-quality IVD antibodies, including this anti-Titin polyclonal antibody, for research and diagnostic development. With a comprehensive portfolio of validated antibody pairs, monoclonal and polyclonal options, and bulk ancillary reagents, CamelBio supports your titin-related projects in muscle biology, cardiomyopathy, and muscular dystrophy. Our expert team also assists in sourcing rare-target raw materials and custom antibody development to accelerate your assays from bench to clinic.