Anti-IFRD1 Rabbit Polyclonal Antibody for WB, ELISA - O00458

Core Product Specifications and Parameters

Biological Background: IFRD1 Function and Localization

• IFRD1 (Interferon-related developmental regulator 1), also known as PC4 or TIS7, is a nerve growth factor (NGF)-inducible protein. The gene undergoes alternative splicing, producing multiple isoforms.
• The protein is proposed to regulate gene activity in the proliferative and differentiative pathways induced by NGF, acting as an autocrine factor that may attenuate or amplify the initial ligand-induced signal (By similarity).
• IFRD1 is broadly expressed in a variety of tissues, consistent with its role in fundamental growth and differentiation processes.
• Classified as a developmental protein and differentiation marker, IFRD1 has been identified through proteomic studies in multiple human tissues.

Experimental Guidance and Technical Tips

• For Western blot, expect a band near 50 kDa for full-length IFRD1. Use knockdown or knockout controls to confirm specificity.
• The immunogen (aa 1-240) may recognize multiple IFRD1 isoforms; verify reactivity with relevant recombinant proteins or lysates.
• In ELISA applications, optimize coating and detection conditions to achieve optimal signal-to-noise ratios.
• Given cross-reactivity with mouse and rat, validate the antibody in the appropriate model system to ensure quantitative accuracy.
• As IFRD1 is NGF-inducible, consider stimulating cells with NGF to enhance detection in expression studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering diagnostic manufacturers and labs seamless access to validated antibodies and ancillary reagents. This IFRD1 antibody supports research into neurotrophin signaling and developmental pathways. For your assay development needs, CamelBio provides everything from monoclonal/polyclonal antibodies and antigen pairs to customized sourcing of rare target reagents, helping you move from concept to clinic with confidence.