Anti-TOLLIP Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9H0E2

Core Product Specifications and Parameters

Biological Background: TOLLIP Function and Localization

• TOLLIP is a key component of the IL-1 and Toll-like receptor signaling pathways, functioning as a negative regulator by inhibiting cell activation upon microbial stimulation. It recruits IRAK1 to the IL-1 receptor complex and blocks IRAK1 phosphorylation and kinase activity. Related references: PMID:10854325 PMID:11751856

• By acting as a ubiquitin-ATG8 family adapter, TOLLIP links the ubiquitin pathway to autophagy, mediating the selective autophagic clearance of ubiquitin conjugates and cytotoxic polyQ protein aggregates. Related references: PMID:25042851

• In conjunction with TOM1, TOLLIP facilitates the recruitment of ubiquitin-conjugated proteins to early endosomes, thereby playing a role in endosomal trafficking. Related references: PMID:15047686

• TOLLIP specifically binds to phosphatidylinositol 3-phosphate (PtdIns(3)P), a lipid enriched on early endosomes and autophagosomes, further supporting its endosomal and autophagic functions. Related references: PMID:26320582

• The protein is predominantly localized in the cytoplasm and on early endosomes, consistent with its roles in signaling and membrane trafficking.

• Post-translational modifications include acetylation and phosphorylation, and the gene undergoes alternative splicing. These modifications may regulate its diverse functions in immunity and autophagy.

• Molecular weight is approximately 30kDa (calculated 30.28 kDa), and it belongs to a family of proteins involved in inflammation and innate immunity.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 1-274 of human TOLLIP, which contains the CUE domain and the TOM1-binding domain. This design may confer reactivity to multiple isoforms and across species (human, mouse, rat).
• For Western blotting, consider using whole cell or tissue lysates that express TOLLIP, such as immune cells or tissues with active autophagy. The predicted band size is ~30kDa.
• In immunohistochemistry (paraffin) and immunofluorescence/immunocytochemistry, fixation and permeabilization conditions should be optimized. Positive staining is expected in punctate cytoplasmic and endosomal patterns.
• For ELISA applications, this polyclonal antibody can be used as a capture or detection reagent after proper titration.
• Always include appropriate controls (e.g., knockout or siRNA knockdown samples) to confirm specificity, especially for close homologs.
• As a polyclonal antibody, lot-to-lot variability may exist; validation in the specific assay system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop partner for IVD raw materials, offering a comprehensive portfolio including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers and manufacturers investigating innate immunity, TLR signaling, or autophagy pathways like those involving TOLLIP, our supply chain solutions ensure reliable access to high-quality reagents and support from concept to clinical stages.