Anti-IFIT2 Polyclonal Antibody for WB, ELISA - P09913

Core Product Specifications and Parameters

Biological Background: IFIT2 Function and Localization

• IFIT2, also known as ISG54 or P54, is an interferon-induced protein belonging to the IFIT family, characterized by tetratricopeptide repeats (TPRs) that mediate antiviral innate immunity.
• It acts as a sensor distinguishing self from non-self mRNA by specifically inhibiting translation of viral mRNAs lacking 2'-O-methylation at the 5' cap.
• The protein binds AU-rich viral RNAs regardless of 5' triphosphorylation status; RNA binding is essential for its antiviral function.
• IFIT2 can promote apoptosis, contributing to the clearance of infected cells.
• Subcellularly, IFIT2 is localized to the cytoplasm and endoplasmic reticulum.
• Proteomic analyses have identified acetylation as a post-translational modification of IFIT2, though its functional significance remains to be fully determined.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 273–472 of human IFIT2, targeting the C-terminal region; this design may provide reactivity against full-length and certain truncated forms.
• For Western blot, expect a band near 55 kDa; post-translational modifications may cause minor shifts. Consider validating the antibody in your sample system with appropriate positive and negative controls.
• As a polyclonal antibody, it may offer high sensitivity but could require careful titration to minimize background. For ELISA, preliminary checkerboard titrations are recommended to optimize coating and detection conditions.
• Cross-reactivity is reported only for human; if working with other species, verify homology of the immunogen region and perform species-specific validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets. For IFIT2 and related interferon-induced proteins, we offer a range of research and diagnostic raw materials to support your immunoassay development.