TFPI Rabbit Polyclonal Antibody for WB, ELISA - P10646

Core Product Specifications and Parameters

Biological Background: TFPI Function and Localization

• Tissue factor pathway inhibitor (TFPI), also known as extrinsic pathway inhibitor (EPI) and lipoprotein-associated coagulation inhibitor (LACI), is a critical regulator of the coagulation cascade.
• TFPI directly inhibits factor Xa and, in a Xa-dependent manner, potently inhibits the VIIa/tissue factor catalytic complex, likely by forming a quaternary Xa/TFPI/VIIa/TF complex.
• The protein possesses antithrombotic properties and can associate with lipoproteins in plasma, contributing to its role in vascular hemostasis.
• TFPI is primarily expressed in endothelial cells, where it is strategically located to prevent thrombosis at the vessel wall.
• The protein is secreted into the bloodstream and also found associated with microsome membranes, indicating a dual localization pattern.
• TFPI contains multiple Kunitz-type serine protease inhibitor domains, is glycosylated, and can be anchored to cell membranes via a GPI anchor, with alternative splicing generating distinct isoforms.
• As a key hemostatic factor, TFPI balances the coagulation system by inhibiting the initiation of the extrinsic pathway.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 29–224 of mature human TFPI, encompassing the N-terminal region critical for factor Xa binding and inhibitory function.
• For Western blot, the predicted molecular weight is ~35 kDa; however, glycosylation or other modifications may cause band shifts. Consider using endothelial cell lysates or recombinant TFPI as positive controls.
• In ELISA, this polyclonal antibody can be used as a capture or detection reagent. Due to cross-reactivity with mouse and rat TFPI, it may be suitable for cross-species studies, but independent validation is advised.
• As a secreted protein that also associates with lipoproteins, sample preparation (e.g., plasma or conditioned medium) may require optimization to ensure accurate detection.
• Being a polyclonal antibody, lot-to-lot variability is possible. Titration and validation in your specific experimental system are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a streamlined bridge from concept to clinic. This anti-TFPI polyclonal antibody is part of our extensive portfolio for coagulation and hemostasis research. Beyond single antibodies, we offer validated antibody pairs, optimized monoclonal reagents, bulk ancillary materials, and expert sourcing assistance for rare targets—enabling a complete, one-stop solution for your IVD raw material needs.