Anti-eIF2A Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9BY44

Core Product Specifications and Parameters

Biological Background: eIF2A Function and Localization

• Eukaryotic translation initiation factor 2A (eIF2A), also known as 65 kDa eIF2A, is encoded by the EIF2A gene and known by aliases CDA02, EIF-2A, MST089, MSTP004, MSTP089.
• Functions in the early steps of protein synthesis by directing the binding of methionyl-tRNAi to 40S ribosomal subunits in a codon-dependent manner, distinct from the GTP-dependent eIF-2 complex.
• Acts on a small number of specific mRNAs, suggesting a role in selective translation initiation.
• Widely expressed in human tissues, with higher levels in pancreas, heart, brain, and placenta.
• Contains WD repeats and coiled coil domains, indicating involvement in protein-protein interactions.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity.
• Alternative splicing generates multiple isoforms, potentially expanding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 450-550) is conserved across human, mouse, and rat eIF2A, supporting the observed cross-reactivity. Verify sequence alignment in your target species.
• For Western blotting, a band at ~65 kDa is expected. Optimize blocking and antibody concentration using lysates from high-expressing tissues such as pancreas or brain.
• In IHC-P, consider antigen retrieval methods and validate staining in known positive tissues (e.g., placenta or brain).
• For ELISA, titrate the antibody against the immunogen peptide or recombinant protein to establish working concentrations.
• As a rabbit monoclonal antibody, it offers high specificity and lot-to-lot consistency. Confirm performance in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic partner for IVD raw material sourcing, providing validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research institutes. Our eIF2A antibody supports translation initiation research and applications in oncology, neuroscience, and metabolic disease. Leverage our one-stop procurement services to streamline your development from concept to clinic.