Anti-eIF3e Rabbit Polyclonal Antibody for WB, ELISA - P60228

Core Product Specifications and Parameters

Biological Background: eIF3e Function and Localization

• eIF3e, also known as INT6, EIF3S6, or eIF3-p48, is a ~52 kDa subunit of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. Related references: PMID:17581632, PMID:25849773, PMID:27462815
• The eIF-3 complex associates with the 40S ribosome to form the 43S pre-initiation complex, stimulates mRNA recruitment and scanning for the AUG start codon, and is required for disassembly and recycling of post-termination complexes, preventing premature subunit joining. Related references: PMID:17581632
• eIF-3 selectively targets a subset of mRNAs involved in cell proliferation, differentiation, and apoptosis, employing RNA stem-loop binding to achieve translational activation or repression. Related references: PMID:25849773
• eIF3e participates in nonsense-mediated mRNA decay (NMD), possibly in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. Related references: PMID:17468741
• eIF3e may interact with MCM7 and EPAS1 and regulate their proteasome-mediated degradation. Related references: PMID:17310990, PMID:17324924
• Subcellularly, eIF3e localizes to the cytoplasm, nucleus, and PML bodies; it is ubiquitously expressed with highest levels in appendix, lymph, pancreas, skeletal muscle, spleen, and thymus.
• Post-translational modifications include acetylation and phosphorylation (from UniProt Keywords).

Experimental Guidance and Technical Tips

• The immunogen covers nearly the full-length protein (amino acids 1-445), which may aid detection of full-size and truncated forms in denaturing Western blot; consider using molecular weight markers and positive controls from human or mouse lysates.
• For Western blot, a band around 52 kDa is expected; however, post-translational modifications or degradation could yield additional bands. Validate specificity with siRNA knockdown or knockout controls if available.
• In ELISA, optimize coating and blocking conditions to minimize non-specific binding; include controls to confirm cross-reactivity with related eIF-3 subunits if necessary.
• Cross-reactivity is verified for human and mouse; test reactivity in other species before experimental use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials, including validated antibodies for translational control research. The eIF3e rabbit polyclonal antibody supports your protein synthesis and disease mechanism studies, and our catalog spans validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your assay development from concept to clinic.