Anti-TFPI2 Polyclonal Antibody for WB, IHC-P, ELISA - P48307

Core Product Specifications and Parameters

Biological Background: TFPI2 Function and Localization

• TFPI2, also known as placental protein 5 (PP5) or TFPI-2, is a secreted serine protease inhibitor belonging to the tissue factor pathway inhibitor family.
• It plays a role in the regulation of plasmin-mediated matrix remodeling and inhibits trypsin, plasmin, factor VIIa/tissue factor complex, and weakly factor Xa; it does not affect thrombin activity.
• The protein contains a signal peptide, multiple disulfide bonds, and glycosylation sites, consistent with its secreted nature.
• It is expressed in umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy.
• Alternative splicing produces multiple isoforms, and the canonical sequence has a molecular weight of approximately 27 kDa.
• Keywords associated with TFPI2 include blood coagulation, hemostasis, protease inhibitor, and 3D-structure, reflecting its role in coagulation and matrix remodeling pathways.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 23-235 of human TFPI2, which includes the mature secreted form; this design supports detection of both precursor and mature protein.
• For Western blotting, a band at approximately 27 kDa is expected, but post-translational modifications like glycosylation may cause a shift; validate in the relevant sample system.
• In immunohistochemistry (paraffin sections), antigen retrieval may be required; consider testing citrate or EDTA-based buffers.
• The antibody cross-reacts with mouse TFPI2, making it suitable for mouse model studies; however, confirm reactivity in your specific tissue or cell line.
• For ELISA, pair with a suitable detection antibody to maximize sensitivity; preliminary checkerboard titrations are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibodies, antigen pairs, and ancillary reagents for coagulation and protease inhibitor research. For TFPI2 and related targets, we offer procurement support for rare raw materials and customized antibody development.