[KD Validated] Anti-eIF4E Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P06730

Core Product Specifications and Parameters

Biological Background: eIF4E Function and Localization

• eIF4E (encoded by EIF4E; aliases EIF4EL1, EIF4F) is a 25 kDa mRNA cap-binding protein and the essential cap-recognition subunit of the eIF4F translation initiation complex, which drives cap-dependent translation by facilitating the recruitment of the 43S pre‑initiation complex to the mRNA 5′ end. Related references: PMID:11606200 PMID:22578813 PMID:22684010
• It specifically binds the 7‑methylguanosine (m7G) cap structure of mRNAs and promotes ATP‑dependent unwinding of 5′‑terminal secondary structures, a critical step for ribosome binding and scanning. Related references: PMID:16271312 PMID:22578813
• In the nucleus, eIF4E mediates mRNA export by forming complexes with LRPPRC and XPO1; it facilitates the nucleocytoplasmic transport of specific transcripts such as NOS2/iNOS and MDM2, thereby influencing their cytoplasmic expression. Related references: PMID:23471078 PMID:22684010
• In cytoplasmic P‑bodies, eIF4E participates in a complex that sequesters translationally inactive mRNAs, preventing their degradation and enabling storage or later translational reactivation. Related references: PMID:24335285
• Together with EIF4G1, eIF4E is required for TISU (Translation Initiator of Short 5′ UTR)‑dependent translation, a non‑scanning mechanism that bypasses the requirement for eIF1‑promoted scanning. Related references: PMID:29987188
• eIF4E localizes to multiple subcellular compartments: cytoplasm (including P‑bodies and stress granules) and nucleus (nucleus speckles, nuclear bodies), a distribution that reflects its dual functions in translation and RNA export.
• The protein is post‑translationally modified by acetylation and phosphorylation, which modulate its cap‑binding affinity and interaction with binding partners.
• Dysregulation of eIF4E is associated with autism spectrum disorder and tumorigenesis, often via chromosomal rearrangements or overexpression that alter cellular translation programs.

Experimental Guidance and Technical Tips

• The immunogen lies within the N‑terminal region (aa 1‑100), an area not heavily modified; these antibodies may detect both phosphorylated and unphosphorylated eIF4E, but users should verify specificity in their own systems.
• For Western blot, expect a major band at ~25 kDa; the reported molecular weight of eIF4E is 25 kDa (theoretical ~25.1 kDa), and it may appear as a single or doublet band depending on phosphorylation status.
• In IHC‑P, antigen retrieval conditions (e.g., citrate buffer pH 6.0) should be optimized; positive controls include high eIF4E‑expressing tissues such as testis, proliferating cells, or certain tumor sections.
• For immunoprecipitation, use protein A or G beads suitable for rabbit polyclonal antibodies; cross‑linking the antibody to beads may improve recovery of low‑abundance complexes.
• IF/ICC may reveal both cytoplasmic and nuclear staining; include appropriate permeabilization and blocking steps, and consider co‑staining with P‑body or nuclear markers to assess localization changes.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio serves as a comprehensive one‑stop partner for IVD raw materials, offering a broad spectrum of antibodies against translation initiation factors and mRNA metabolism targets. Our portfolio includes validated polyclonal antibodies like this anti‑eIF4E, as well as matched monoclonal pairs and ancillary reagents, designed to support diagnostic kit development and life science research. For customized eIF4E‑related raw materials or bulk orders, please contact our sourcing team to discuss your specific requirements.