[KO Validated] IFITM3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q01628

Core Product Specifications and Parameters

Biological Background: IFITM3 Function and Localization

• IFITM3 is an interferon-induced antiviral protein that restricts the entry of a broad range of enveloped viruses, including influenza A virus, SARS-CoV, SARS-CoV-2, Ebola virus, and HIV-1, by inhibiting viral fusion with cholesterol-depleted endosomes (PubMed:26354436; PubMed:33239446; PubMed:33270927). Related references: PMID:26354436 PMID:33239446 PMID:33270927
• Belongs to the dispanin family (DSPA2b) and is also known as 1-8U or IP15, with interferon-inducible expression.
• Plays a critical role in the structural stability and function of vacuolar ATPase (v-ATPase) on endosomes, facilitating proper clathrin localization and endosomal acidification required for its antiviral activity (PubMed:26354436). Related references: PMID:26354436
• In hepatocytes, cooperates with IFITM1 and IFITM2 to restrict hepatitis C virus infection by targeting endocytosed virions for lysosomal degradation (PubMed:26354436). Related references: PMID:26354436
• Subcellularly localizes to the plasma membrane, late and early endosome membranes, lysosome membranes, and the perinuclear cytoplasm.
• Undergoes key post-translational modifications including palmitoylation, phosphorylation, and ubiquitination, which modulate its antiviral potency and stability.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-133 of human IFITM3, which includes the N-terminal region. For Western blotting, a band around 15 kDa is expected; validate in a relevant interferon-stimulated cell line.
• For IHC-P and IF/ICC, consider optimizing antigen retrieval and permeabilization steps, as IFITM3 is an integral membrane protein requiring membrane permeabilization for detection.
• Cross-reactivity with mouse and rat IFITM3 is listed; however, sequence alignment and pilot experiments in these species are recommended to confirm antibody performance.
• This antibody is validated for ELISA; it may be suitable for sandwich ELISA development when paired with a detection antibody recognizing a non-overlapping epitope.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes by providing one-stop access to IVD raw materials, spanning from early-stage research to clinical applications. For projects targeting antiviral innate immunity and interferon signaling, such as those involving IFITM3, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare-target raw materials. Our integrated supply chain streamlines development pipelines, helping you accelerate the transition from concept to clinic.