Anti-IFITM3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q01628

Core Product Specifications and Parameters

Biological Background: IFITM3 Function and Localization

• IFITM3 (Interferon-induced transmembrane protein 3) is a member of the interferon-inducible transmembrane (IFITM) protein family, also known as dispanin subfamily A member 2b (DSPA2b) or 1-8U.
• It functions as an IFN-induced antiviral protein that disrupts intracellular cholesterol homeostasis, inhibiting viral entry by preventing fusion with cholesterol-depleted endosomes. Related references: PMID:26354436 PMID:33239446 PMID:33270927
• Active against a broad range of viruses including influenza A, SARS coronaviruses (SARS-CoV and SARS-CoV-2), Ebola, Marburg, Dengue, West Nile, HIV-1, HCV, and VSV. Related references: PMID:26354436 PMID:33239446 PMID:33270927
• Plays a critical role in vacuolar ATPase (v-ATPase) structural stability and function, establishing endosomal pH necessary for the antiviral state. Related references: PMID:26354436
• Subcellular localization includes cell membrane, late endosome membrane, early endosome membrane, lysosome membrane, and perinuclear cytoplasm.
• Post-translational modifications include palmitoylation, phosphorylation, and ubiquitination, which may regulate its antiviral activity.
• In hepatocytes, coordinates with other IFITM proteins to restrict HCV infection by targeting virions for lysosomal degradation. Related references: PMID:26354436

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal amino acids 1–57 of human IFITM3, suggesting the antibody may recognize an epitope in this region.
• For Western blot (WB), the predicted molecular weight is approximately 15 kDa; however, post-translational modifications may cause shifts, so validate in the relevant sample system.
• IFITM3 expression is interferon-inducible; consider using interferon-treated human cell lines or tissues to enhance detection sensitivity.
• For IHC-P, optimize antigen retrieval and antibody dilution using appropriate controls, as detection may depend on expression levels and tissue processing.
• While validated for human reactivity, cross-reactivity with other species or IFITM family members is possible; verify in the intended experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for diagnostic manufacturers and research laboratories, delivering validated IVD raw materials and technical support from concept to clinic. Our product portfolio includes high-quality monoclonal antibodies like this IFITM3 Rabbit mAb, suitable for antiviral research and innate immunity studies. In addition to individual reagents, we supply optimized antibody pairs, bulk ancillary reagents, and can assist in sourcing rare-target raw materials to accelerate your assay development.