Anti-TFAM Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q00059

Core Product Specifications and Parameters

Biological Background: TFAM Function and Localization

• TFAM (Transcription Factor A, Mitochondrial), also known as MtTF1, TCF-6, and TCF6L2, is a key nuclear-encoded mitochondrial transcription factor that regulates mitochondrial gene expression.
• It forms the mitochondrial transcription initiation complex with TFB2M and POLRMT, essential for basal transcription of mitochondrial DNA (mtDNA).
  Related references: PMID:29149603
• TFAM binds to the light strand and HSP1 promoters, bending the DNA into a U-turn via its HMG-box domains to recruit POLRMT and facilitate transcription initiation.
  Related references: PMID:22037172, PMID:1737790
• Beyond transcription, TFAM is critical for mtDNA maintenance and packaging; it compacts mtDNA into nucleoids, and its dosage tightly regulates mtDNA copy number.
  Related references: PMID:19304746, PMID:22841477, PMID:22037171
• In cooperation with TFB2M, TFAM enables promoter opening and trapping of the DNA non-template strand, a crucial step for accurate transcription.
  Related references: PMID:20410300
• TFAM localizes to the mitochondrial matrix and nucleoids, where it also plays a role in unwinding and organizing mtDNA.
• It undergoes alternative splicing and phosphorylation; disease-associated variants are linked to primary mitochondrial disorders.
• As a member of the HMGB family, TFAM uses its tandem HMG boxes to bind, bend, and loop mtDNA, contributing to nucleoid architecture.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1–220 region of human TFAM, which includes the tandem HMG-box domains; thus, the antibody is expected to recognize full-length TFAM.
• For Western blotting, consider using mitochondrial-enriched lysates for optimal detection; the predicted molecular weight is ~29 kDa, but post-translational modifications may cause a shift.
• For immunofluorescence/IF and ICC, anticipate a mitochondrial punctate staining pattern; co-staining with a mitochondrial marker is recommended to confirm localization.
• When performing ELISA, titrate the antibody against recombinant TFAM or cell lysates to establish optimal working dilutions.
• Due to high sequence conservation across human, mouse, and rat, cross-reactivity is likely, but validate the antibody in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to premium IVD raw materials, including validated antibody pairs and bulk ancillary reagents. This anti-TFAM polyclonal antibody exemplifies our commitment to delivering high-quality reagents for mitochondrial research and assay development. Contact our team to explore customized sourcing solutions for your next project.