Anti-Tafazzin / TAZ Rabbit Polyclonal Antibody for WB, ELISA - Q16635

Core Product Specifications and Parameters

Biological Background: Tafazzin / TAZ Function and Localization

• Tafazzin (TAZ), encoded by the TAFAZZIN gene and also known as protein G4.5, is a mitochondrial acyltransferase expressed as multiple isoforms via alternative splicing, with tissue-specific patterns.
• Catalyzes the remodeling of cardiolipin (CL) in the mitochondrial inner membrane through CoA-independent transacylation between phospholipids, primarily phosphatidylcholine (PC) and CL. This activity is critical for respiratory chain complex assembly, stability, and overall mitochondrial function. Related references: PMID:12930833 PMID:19164547 PMID:19700766 PMID:32234310
• Required for the initiation of mitophagy, linking CL remodeling to mitochondrial quality control. Related references: PMID:33096711
• Localizes to the mitochondrial outer membrane, inner membrane, and cytoplasm, consistent with its dynamic role in phospholipid metabolism and membrane dynamics.
• Highly expressed in cardiac and skeletal muscle; mutations cause Barth syndrome (BTHS), an X-linked cardiomyopathy. Tissue-specific isoforms exist, with N-terminus-lacking variants found in leukocytes and fibroblasts but not in heart or skeletal muscle.
• Essential for spermatogenesis, including spermatid individualization and meiotic progression (by similarity).
• Keywords: Acyltransferase, Alternative splicing, Cardiomyopathy, Lipid metabolism, Mitochondrion, Transferase.

Experimental Guidance and Technical Tips

• For Western blotting, consider using mitochondrial-enriched lysates from heart or skeletal muscle tissue to enhance detection of endogenous Tafazzin.
• Validate cross-reactivity in mouse and rat samples, as the immunogen shares significant homology with these species.
• Due to alternative splicing, multiple bands may be observed near the predicted 30 kDa; confirm isoform identity via molecular weight markers or isoform-specific controls.
• For ELISA, use a recombinant Tafazzin protein standard to establish assay sensitivity and linear range.
• Optimize antigen retrieval and blocking conditions if testing tissue sections, as mitochondrial membrane proteins can be challenging.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. This Tafazzin/TAZ antibody is ideally suited for studies in mitochondrial biology, cardiac muscle function, and Barth syndrome research. Contact us to explore custom procurement options and accelerate your project from concept to clinic.