Anti-IFNL1 Polyclonal Antibody for WB, IHC-P, ELISA - Q8IU54

Core Product Specifications and Parameters

Biological Background: IFNL1 Function and Localization

• Interferon lambda-1 (IFNL1), also known as Cytokine Zcyto21 or Interleukin-29 (IL-29), is a member of the type III interferon family.
• Functions as a cytokine with antiviral, antitumor, and immunomodulatory activities, playing a critical role in antiviral host defense, predominantly in epithelial tissues.
• Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, activating the JAK/STAT signaling pathway to induce expression of interferon-stimulated genes (ISG) that mediate an antiviral state.
• Its activity is primarily restricted to epithelial cells due to the epithelial cell‑specific expression of its receptor IFNLR1.
• Exerts immunomodulatory effects by up‑regulating MHC class I antigen expression.
• Subcellular location: secreted from cells via a signal peptide.
• Key structural features include disulfide bonds and glycosylation; identified through proteomics and 3D‑structure determination.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 20–200 of human IFNL1, corresponding to the mature secreted protein; this design supports detection of endogenous IFNL1.
• For Western blot, expect a band near 22 kDa under reducing conditions; validate expression in relevant epithelial cell lines or stimulated tissues.
• In IHC‑P, optimize antigen retrieval and blocking; signal may be observed in epithelial cells due to the restricted receptor expression.
• ELISA applications can be developed using this antibody; consider pairing with a matched detection antibody for increased sensitivity.
• Cross‑reactivity with mouse and rat IFNL1 allows use in animal models, but confirm sequence homology to ensure consistent reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti‑IFNL1 polyclonal antibody is part of CamelBio’s extensive portfolio for cytokine and antiviral research, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. CamelBio also specializes in sourcing rare‑target raw materials, ensuring reliable supply for your IVD assay development needs.