Anti-SR-BI Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q8WTV0

Core Product Specifications and Parameters

Biological Background: SR-BI Function and Localization

• SR-BI, also known as CLA-1, is a member of the CD36 scavenger receptor family and functions as a receptor for various ligands including lipoproteins, phospholipids, and apoptotic cells.
• It mediates selective uptake of cholesteryl esters from high-density lipoprotein (HDL) and facilitates cellular cholesterol efflux, playing a critical role in reverse cholesterol transport. Related references: PMID:26965621
• Through its phosphatidylserine binding activity, SR-BI participates in the phagocytosis of apoptotic cells. Related references: PMID:12016218
• SR-BI acts as a host cell receptor for hepatitis C virus (HCV) by binding to envelope glycoprotein E2, facilitating viral entry independently of genotype. Related references: PMID:12356718, PMID:12913001, PMID:18000990
• Additionally, SR-BI mediates bacterial uptake and serves as an entry cofactor for SARS-CoV-2 through an HDL-dependent mechanism.
• The protein is localized on the cell membrane and within caveolae, and is widely expressed in human tissues. It undergoes co- and post-translational modifications such as N-glycosylation and palmitoylation, which may influence its function and trafficking.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human SR-BI. For western blotting, the predicted molecular weight is ~61 kDa, but a higher band may be observed due to glycosylation; consider using deglycosylation enzymes if precise size determination is required.
• For immunohistochemistry (IHC-P), antigen retrieval methods such as heat-induced epitope retrieval may enhance detection. Validate antibody specificity using appropriate positive and negative tissue controls.
• The antibody has reported cross-reactivity with human, mouse, and rat samples. When working with these species, ensure that the target epitope sequence is conserved.
• For ELISA applications, pairing with other clones may be necessary; optimize coating and detection conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research institutes from concept to clinic. Our SR-BI antibody portfolio aids in studying lipid metabolism, viral pathogenesis, and scavenger receptor biology. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development and production scale-up.