Anti-B6R Rabbit Monoclonal Antibody for WB, ELISA - Q8V4S2

Core Product Specifications and Parameters

Biological Background: B6R Function and Localization

• Protein OPG190, also known as B6R or plaque-size/host range protein, is a key factor in monkeypox virus morphogenesis and entry.
• It facilitates the dissolution of the outermost membrane of extracellular enveloped virions (EV), enabling viral entry into host cells.
• B6R participates in wrapping intracellular mature virions (MV) to form enveloped virions, a critical step in viral egress.
• The protein localizes to the host Golgi apparatus and trans-Golgi network, as well as the virion membrane.
• B6R contains a signal peptide, a Sushi domain (also known as CCP/SCR), and a transmembrane helix, indicating it is membrane-associated.
• As an early protein, B6R is expressed early in the viral life cycle, consistent with its role in virion assembly.
• Its disulfide bond features suggest structural stability important for its function at the host-virus interface.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20-279, covering the predicted ectodomain of B6R; consider using similar regions for capture/detection in ELISA setups.
• For Western blot, reduce and denature samples to detect a band around 35 kDa; validate in Monkeypox virus (strain Zaire-96-I-16) infected cell lysates.
• This antibody has been tested for WB and ELISA; for other applications, users should optimize dilution and buffer conditions experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This anti-B6R rabbit monoclonal antibody supports research into monkeypox virus entry and maturation, empowering diagnostic and vaccine development workflows. Our platform bridges the gap from concept to clinic with comprehensive technical services and consulting.