Anti-SR-BI Rabbit Polyclonal Antibody for WB, ELISA - Q8WTV0

Core Product Specifications and Parameters

Biological Background: SR-BI Function and Localization

• SR-BI is a multiligand scavenger receptor that binds phospholipids, cholesterol esters, lipoproteins (HDL, LDL, VLDL), phosphatidylserine, and apoptotic cells, thereby participating in lipid metabolism and clearance of apoptotic debris. Related references: PMID:12016218 PMID:12519372 PMID:21226579
• It serves as the primary receptor for high-density lipoprotein (HDL), mediating selective cholesteryl ester uptake into cells and promoting cholesterol efflux, a crucial step in reverse cholesterol transport. Related references: PMID:26965621
• Through its phosphatidylserine-binding activity, SR-BI contributes to the recognition and phagocytosis of apoptotic cells. Related references: PMID:12016218
• SR-BI acts as a key entry factor for hepatitis C virus (HCV) by interacting with the viral envelope glycoprotein E2, irrespective of viral genotype. Related references: PMID:12356718 PMID:12913001 PMID:18000990
• Additionally, SR-BI facilitates cellular entry of SARS-CoV-2 and mediates the internalization of bacteria such as Mycobacterium fortuitum, Escherichia coli, and Staphylococcus aureus.
• Subcellularly, SR-BI localizes to the plasma membrane and caveolae, where it forms clusters that facilitate lipid exchange.
• The protein is subject to post-translational modifications including N-glycosylation, palmitoylation, and disulfide bond formation, which regulate its trafficking and function.

Experimental Guidance and Technical Tips

• The immunogen, a synthetic peptide spanning residues 1–100 within the N-terminal extracellular region of human SR-BI, is likely to generate antibodies recognizing the native receptor on the cell surface.
• For Western blot (WB), this rabbit polyclonal antibody is expected to detect a band at ~61 kDa (predicted molecular weight) under reducing conditions; however, due to glycosylation, the observed band may shift higher in some cell lysates.
• In ELISA applications, the antibody can be employed for antigen capture or detection, but optimal dilutions should be determined experimentally.
• Cross-reactivity with mouse and rat SR-BI is reported, allowing its use in rodent studies; however, species-specific validation is recommended.
• Antibody specificity should be confirmed using positive and negative control lysates (e.g., SR-BI-overexpressing cells vs. knockout cells) to account for any nonspecific binding inherent to polyclonal preparations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including this anti-SR-BI polyclonal antibody for cholesterol metabolism and viral entry studies. Our comprehensive portfolio covers validated antibody pairs, recombinant proteins, and bulk ancillary reagents, supporting everything from assay development to scale-up production. For rare or customized targets, CamelBio’s expert sourcing team ensures reliable supply to meet your project needs.