Anti-Survivin Rabbit Monoclonal Antibody for WB, ELISA - O15392

Core Product Specifications and Parameters

Biological Background: Survivin Function and Localization

• Survivin (BIRC5) is a dual-function protein that promotes cell proliferation and inhibits apoptosis. Related references: PMID:20627126 PMID:21364656 PMID:25778398
• As a core component of the chromosomal passenger complex (CPC), it is essential for chromosome alignment, segregation, and cytokinesis. Related references: PMID:16322459
• It directs CPC movement from inner centromeres during prometaphase to the midbody during cytokinesis and associates with polymerized microtubules to organize the central spindle. Related references: PMID:20826784
• Survivin recruits CPC to centromeres by binding histone H3 phosphorylated at Thr-3 (H3pT3), and its complex with RAN delivers TPX2 to microtubules for mitotic spindle formation. Related references: PMID:20929775 PMID:18591255
• It counteracts apoptosis induction in G2/M phase and directly inhibits caspase-3 and caspase-7. Related references: PMID:9859993 PMID:21536684
• The acetylated form represses STAT3 transactivation, and it maintains mitochondrial integrity and function. Related references: PMID:20826784 PMID:25778398
• Isoforms 2 and 3 are not critical for mitosis, and isoform 3 shows markedly reduced anti-apoptotic effects. Related references: PMID:12773388 PMID:16291752 PMID:10626797
• Subcellularly, Survivin localizes to the cytoplasm, nucleus, centromeres, kinetochores, spindle, and midbody, and is overexpressed in various cancers while restricted to fetal kidney/liver and cochlea in normal tissues.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-142 of human Survivin, covering the full-length protein; the antibody may detect all isoforms, but validation with specific isoform controls is recommended.
• For WB, use reducing conditions and a high-percentage gel (e.g., 12–15%) to resolve the ~16 kDa Survivin band. Include positive controls from human cancer cell lines (e.g., HeLa) known to express Survivin.
• Survivin expression peaks at G2/M phase; cell synchronization may enhance detection in cycling cultures.
• For ELISA, consider pairing with a capture antibody targeting a distinct epitope to avoid competition, and validate in the matrix of interest.

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