Anti-SIRT2 Rabbit Monoclonal Antibody for WB, ELISA - Q8IXJ6

Core Product Specifications and Parameters

Biological Background: SIRT2 Function and Localization

• NAD-dependent protein deacetylase that removes acetyl groups from internal lysines on histones, α-tubulin, and transcription factors, regulating diverse processes including cell cycle, microtubule dynamics, and metabolism. Related references: PMID:12620231 PMID:16648462 PMID:18249187
• Key cell cycle regulator: deacetylates CDC20 and FZR1 to activate the APC/C ubiquitin ligase, controls antephase checkpoint in response to microtubule stress, and modulates histone H4 Lys-20 methylation during mitosis. Related references: PMID:12697818 PMID:16909107 PMID:17488717 PMID:22014574 PMID:23468428
• Localizes to multiple subcellular compartments including nucleus, cytoplasm, centrosome, spindle, midbody, and growth cone, with dynamic shuttling during cell division.
• Metabolic sensor: deacetylates and activates G6PD in the pentose phosphate pathway, stabilizes PCK1 in gluconeogenesis, and promotes lipolysis while inhibiting adipogenesis via FOXO1-PPARG axis. Related references: PMID:20543840 PMID:21726808 PMID:24769394
• Tumor suppressor: down-regulated in gliomas and several cancers; deacetylates p53, HIF1A, and RELA to inhibit oncogenic signaling and hypoxia response. Related references: PMID:22014574 PMID:18249187 PMID:18995842 PMID:24681946 PMID:21081649
• Possesses demyristoylase and depalmitoylase activity toward targets like KRAS and ARF6, regulating membrane association and signaling. Related references: PMID:25704306 PMID:29239724 PMID:32103017

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 of human SIRT2 (Q8IXJ6). This N-terminal region may be conserved across isoforms; validate detection of specific splice variants if required.
• Recommended applications: Western blot (WB) and ELISA. For WB, a band at ~43 kDa is expected; consider using whole cell lysates from human cell lines (e.g., HeLa, HEK293) as positive controls.
• For ELISA, optimize coating antigen and antibody dilution based on your assay format; the peptide immunogen can serve as a positive control.
• SIRT2 localizes to both nucleus and cytoplasm; fractionation assays may aid in studying compartment-specific functions.
• Post-translational modifications (e.g., phosphorylation at Ser-368) may affect antibody binding; verify reactivity in relevant physiological conditions.

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