[KO Validated] Anti-SIRT1 Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q96EB6

Core Product Specifications and Parameters

Biological Background: SIRT1 Function and Localization

• SIRT1 is an NAD-dependent protein deacetylase that couples transcriptional regulation to cellular energy status, influencing cell cycle progression, DNA damage response, metabolism, apoptosis, and autophagy. Related references: PMID:11672523 PMID:12006491 PMID:14976264

• It modulates chromatin structure by deacetylating histones H1, H2A, H3, and H4, promoting transcriptional repression and the formation of silent heterochromatin, particularly at rDNA loci. Related references: PMID:15469825 PMID:18485871

• SIRT1 deacetylates a multitude of transcription factors and co-regulators, including p53, FOXO3, NF-κB, and MYC, thereby regulating stress responses, cell survival, and proliferation. Related references: PMID:11672523 PMID:12006491 PMID:14976264 PMID:15152190 PMID:21807113

• Acting as a sensor of the intracellular NAD+/NADH ratio, SIRT1 mediates metabolic adaptations during caloric restriction, enhances hepatic gluconeogenesis, and promotes fatty acid oxidation in skeletal muscle. Related references: PMID:15205477 PMID:15692560 PMID:23142079

• SIRT1 participates in DNA damage repair by deacetylating key repair proteins such as Ku70, NBN, and WRN, and facilitates the recruitment of repair factors to damaged sites. Related references: PMID:15205477 PMID:16998810 PMID:17334224 PMID:20097625

• It plays a role in autophagy by deacetylating ATG5, ATG7, and LC3, and helps maintain circadian rhythm through deacetylation of BMAL1 and PER2. Related references: PMID:18296641 PMID:18662546

• Subcellularly, SIRT1 is primarily nuclear, enriched in PML nuclear bodies, but can also translocate to the cytoplasm and mitochondria under specific conditions.

• Widely expressed across human tissues, SIRT1 is critically involved in skeletal muscle differentiation, neuronal development, and cardiovascular protection.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant full-length human SIRT1 protein; therefore, the antibody may recognize all isoforms. For KO validation, ensure the targeted exon is disrupted in your knockout model.
• For Western blot, expect a major band at ~82 kDa. Use appropriate positive and negative controls, including KO cell lysates if available, to confirm specificity.
• In immunofluorescence and IHC, nuclear staining is expected; however, permeabilization and fixation conditions may need optimization due to the antigen’s localization.
• SIRT1 expression is ubiquitous, but levels can vary; consider using loading controls and validating the antibody in your tissue/cell type of interest before quantitative assays.
• This antibody is listed for flow cytometry (intracellular); ensure proper permeabilization and include an isotype control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to premium IVD raw materials, including validated monoclonal antibodies like this anti-SIRT1 antibody for epigenetics and metabolism research. Whether you need optimized antibody pairs, bulk reagents, or customized sourcing for rare targets, our integrated supply chain and technical consulting help accelerate your assay development from concept to clinic.