Anti-Survivin Rabbit Monoclonal Antibody for WB and ELISA - O15392

Core Product Specifications and Parameters

Biological Background: Survivin Function and Localization

• Survivin (Baculoviral IAP repeat-containing protein 5) is a 16 kDa protein belonging to the inhibitor of apoptosis (IAP) family. Isoforms 2 and 3 are expressed but show reduced anti-apoptotic activity, with isoform 3 exhibiting a marked reduction compared to the wild-type. Related references: PMID:12773388 PMID:16291752 PMID:10626797
• It plays a dual role in promoting cell proliferation and inhibiting apoptosis. Related references: PMID:20627126 PMID:21364656 PMID:25778398
• As a core component of the chromosome passenger complex (CPC), survivin is essential for chromosome alignment, segregation, and cytokinesis. Related references: PMID:16322459
• Survivin directs CPC localization from the inner centromere to the midbody and organizes the central spindle via microtubule association. Its acetylated form represses STAT3 target gene transcription. Related references: PMID:20826784
• It recruits CPC to centromeres by binding histone H3 phosphorylated at Thr-3 (H3pT3) during early mitosis. Related references: PMID:20929775
• In a complex with RAN, survivin scaffolds the delivery of TPX2 to microtubules, facilitating mitotic spindle formation. Related references: PMID:18591255
• Survivin counteracts apoptosis in the G2/M phase and directly inhibits caspase-3 and caspase-7. Related references: PMID:9859993 PMID:21536684
• Subcellularly, survivin resides in the cytoplasm, nucleus, centromere, kinetochore, mitotic spindle, and midbody. It is abundantly expressed in fetal tissues and various adenocarcinomas.

Experimental Guidance and Technical Tips

• For Western blot, consider using the recommended loading buffer and validating the signal in human, mouse, or rat cell lysates to confirm specificity.
• Since the immunogen covers the N-terminal region (aa 1–140), the antibody may recognize full-length survivin and possibly some isoforms; validate reactivity in your target sample system.
• ELISA protocols may require optimization of coating antigen and antibody dilution to achieve optimal signal-to-noise ratios.
• The antibody’s performance in other applications not listed has not been tested; consider conducting pilot experiments if deviating from WB or ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-survivin antibody for apoptosis and cell proliferation studies. Our integrated supply chain offers antibody pairs, custom immunogen design, recombinant proteins, and ancillary reagents to streamline assay development. Count on our expertise to source even rare-target raw materials efficiently, accelerating your journey from concept to clinic.