Anti-RAB11A/B Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P62491 / Q15907

Core Product Specifications and Parameters

Biological Background: RAB11A/B Function and Localization

• This antibody recognizes both RAB11A and RAB11B. RAB11A cycles between an inactive GDP-bound and active GTP-bound state, recruiting downstream effectors for vesicle formation, movement, tethering and fusion. Related references: PMID:15601896, PMID:15689490, PMID:17462998

• RAB11A is a master regulator of endocytic recycling, forming a complex with RAB11FIP3 and dynein to move sorting endosomes along microtubules toward the centrosome, generating the endosomal recycling compartment. Related references: PMID:20026645

• RAB11A plays a critical role in membrane delivery during cytokinesis. Related references: PMID:15601896

• Together with MYO5B and RAB8A, RAB11A contributes to epithelial cell polarization and, with Rabin8/RAB3IP and the exocyst, promotes apical membrane formation and lumenogenesis. Related references: PMID:21282656, PMID:20890297

• RAB11A interacts with MYO5B to facilitate CFTR trafficking and transferrin recycling, and with RAB11FIP2 for NPC1L1 plasma membrane delivery. Related references: PMID:17462998, PMID:19542231

• RAB11A regulates sorting and basolateral transport of CDH1 from the Golgi, and participates in ciliogenesis via a ciliary targeting complex with ASAP1, Rabin8, RAB11FIP3 and ARF4. Related references: PMID:15689490, PMID:25673879, PMID:31204173

• Subcellularly, RAB11A localizes to recycling endosomes, the Golgi apparatus, cleavage furrow, phagosome membrane, and cilia, consistent with its diverse trafficking roles.

• Post-translational regulation includes geranylgeranylation for membrane anchoring, and reversible acetylation and methylation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) shared by RAB11A and RAB11B, suggesting the antibody may detect both isoforms. Validate specificity by siRNA knockdown or isoform-specific controls in your sample system.
• For Western blotting (WB), a band around 24 kDa is expected. Consider using reducing conditions and appropriate loading controls.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), RAB11A exhibits vesicular and membrane staining patterns. Optimize permeabilization and fixation protocols to preserve endosomal structures.
• ELISA applications can be used for quantification; cross-reactivity with closely related Rab proteins should be assessed if required.
• The antibody has been validated for human, mouse, and rat samples. Test in other species if sequence homology suggests cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. The RAB11A/B rabbit monoclonal antibody supports research into membrane trafficking, endocytic recycling, and cell polarization—key areas for disease modeling and therapeutic development. With a one-stop vertical chain covering reagent supply, technical consultation, and scale-up solutions, CamelBio accelerates your path from concept to clinic.