Anti-RXRB Rabbit Monoclonal Antibody for WB, IP, ChIP, ELISA - P28702

Core Product Specifications and Parameters

Biological Background: RXRB Function and Localization

• RXRB encodes the retinoic acid receptor RXR-beta, a member of the nuclear receptor superfamily that functions as a ligand-activated transcription factor.
• It forms heterodimers with retinoic acid receptors (RARs) and binds to retinoic acid response elements (RARE) to regulate gene expression in response to all-trans or 9-cis retinoic acid.
• The protein contains conserved DNA-binding zinc-finger motifs, characteristic of nuclear receptors, enabling direct interaction with DNA.
• RXRB localizes to both the nucleus and cytoplasm, where it participates in signal transduction and transcriptional regulation.
• At the protein level, RXRB is expressed in aortic endothelial cells. Related references: PMID:28167758
• It is also detected in monocytes. Related references: PMID:26463675
• The protein is expressed in a variety of tumor cell lines, implicating roles in tumor biology.
• Post-translational modifications such as methylation and alternative splicing generate multiple RXRB isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human RXRB, which may allow detection of multiple isoforms. Confirm band patterns in your system.
• For Western blot, the predicted molecular weight is approximately 57 kDa; use a suitable gel percentage (e.g., 10% SDS-PAGE) and validate with appropriate markers.
• In immunoprecipitation, the rabbit monoclonal format offers high specificity; consider titrating antibody concentration to minimize background.
• For ChIP assays, optimize cross-linking and chromatin fragmentation conditions due to RXRB's dual nuclear/cytoplasmic localization. Validate enrichment by qPCR at known RAREs.
• Confirm cross-reactivity with mouse models by testing lysates from relevant mouse tissues or cell lines.
• Use anti-rabbit secondary antibodies for detection in all applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive selection of IVD raw materials, including validated monoclonal antibodies, recombinant proteins, and ancillary reagents, to accelerate assay development. This anti-RXRB rabbit monoclonal antibody is part of our nuclear receptor portfolio, supporting research in retinoic acid signaling and gene regulation. For unique requirements, we provide sourcing of rare-target antibodies and custom reagent solutions, bridging the gap from discovery to clinical application.