Anti-PDK1/PDHK1 Rabbit Monoclonal Antibody for WB, ELISA - Q15118

Core Product Specifications and Parameters

Biological Background: PDK1/PDHK1 Function and Localization

• PDK1 is a mitochondrial pyruvate dehydrogenase kinase that specifically phosphorylates the E1 alpha subunits (PDHA1 and PDHA2) of the pyruvate dehydrogenase complex, leading to its inactivation. This phosphorylation inhibits the conversion of pyruvate to acetyl-CoA, thereby regulating the tricarboxylic acid cycle and aerobic respiration. Related references: PMID:7499431 PMID:18541534 PMID:22195962 PMID:26942675
• Through PDC inhibition, PDK1 shifts cellular energy metabolism from glucose oxidation to fatty acid oxidation, playing a pivotal role in glucose and fatty acid homeostasis. Related references: PMID:7499431 PMID:18541534 PMID:22195962 PMID:26942675
• PDK1 is highly expressed in the heart, with lower levels in liver, skeletal muscle, and pancreas, tissues with high metabolic demand.
• The kinase localizes to the mitochondrial matrix, consistent with its role in regulating the pyruvate dehydrogenase complex situated in the inner mitochondrial membrane.
• PDK1 is alternatively spliced and contains a mitochondrial transit peptide, ensuring its proper import into mitochondria.
• Under hypoxic conditions, PDK1 is upregulated to down-regulate aerobic respiration, promoting cell survival and proliferation in low-oxygen environments. Related references: PMID:7499431 PMID:18541534 PMID:22195962 PMID:26942675
• The protein is an ATP-binding kinase with phosphoprotein modifications, indicating regulatory post-translational control.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-100 of human PDK1, an N-terminal region that may detect total PDK1 independent of splice variants. Validate detection in your specific sample system.
• For Western blot, expect a band at approximately 49 kDa in human and mouse lysates. Consider using proper positive and negative controls to confirm specificity.
• The antibody is recommended for ELISA; optimize coating antigen and antibody dilution to achieve desired sensitivity and linear range.
• As a rabbit monoclonal antibody, it offers high consistency and reproducibility across experiments and lots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, providing validated antibodies and ancillary reagents for metabolic disease research. This anti-PDK1 monoclonal antibody is indicative of our capability to supply high-quality, application-tested reagents for diagnostic development. Contact us to explore custom sourcing of rare targets or bulk quantities to accelerate your assay pipeline.