Anti-PDGFRB Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P09619

Core Product Specifications and Parameters

Biological Background: PDGFRB Function and Localization

• PDGFRB (Platelet-derived growth factor receptor beta, CD140B) is a receptor tyrosine kinase that binds PDGFB and PDGFD homodimers, as well as PDGFA/PDGFB heterodimers, initiating intracellular signaling cascades critical for cell proliferation, survival, differentiation, and migration.
• It plays an essential role in embryonic development and blood vessel formation by promoting proliferation, migration, and recruitment of pericytes and vascular smooth muscle cells to endothelial cells.
• PDGFRB can heterodimerize with PDGFRA, modulating downstream responses depending on the ligand and dimer composition.
• Upon ligand binding, the receptor autophosphorylates and activates multiple signaling pathways, including PLCG1-calcium-PKC, PI3K-AKT, and RAS-MAPK cascades, by phosphorylating adaptor proteins such as SHC1, GRB2, PTPN11, and others.
• Subcellularly, PDGFRB is located at the cell membrane, and upon activation it is internalized into cytoplasmic vesicles and lysosomes where signaling is downregulated.
• The protein undergoes post-translational modifications including tyrosine phosphorylation, glycosylation, and ubiquitination.
• PDGFRB is implicated in vascular injury response and neointima formation, and mutations or rearrangements are associated with developmental disorders and certain cancers.

Experimental Guidance and Technical Tips

• The antibody was raised against a peptide spanning the C-terminal intracellular region (aa 1000-1106) of human PDGFRB. It may detect the full-length, unprocessed receptor but not secreted or truncated forms lacking this domain.
• For Western blotting, a band of approximately 124 kDa is expected. Consider using reducing conditions and appropriate controls to confirm PDGFRB expression.
• In immunofluorescence/ICC, signal may be observed at the plasma membrane and in intracellular vesicles. Fixation and permeabilization methods should be optimized for the cell type.
• Cross-reactivity with mouse PDGFRB has been reported; however, validation in the specific experimental system is recommended.
• This recombinant rabbit monoclonal antibody offers consistent batch-to-batch reproducibility, suitable for quantitative ELISA applications; appropriate standards and controls should be included.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a reliable, one-stop source for IVD raw materials, including optimized antibodies for targets like PDGFRB. Our portfolio spans validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare or challenging targets, supporting assay development from concept to clinical application. Contact us to streamline your procurement and accelerate your programs in angiogenesis and receptor tyrosine kinase research.