Anti-PLK1 Rabbit Monoclonal Antibody (PLK1/STPK13) for WB, ELISA - P53350

Core Product Specifications and Parameters

Biological Background: PLK1 Function and Localization

• PLK1 (Polo-like kinase 1, STPK13) is a serine/threonine-protein kinase that acts through POLO box domains to bind and phosphorylate pre-phosphorylated substrates, coordinating multiple mitotic events.
• It performs essential M-phase functions: centrosome maturation, spindle assembly, cohesin removal, APC/C inactivation, and mitotic exit/cytokinesis. Related references: PMID:11202906 PMID:12207013 PMID:12447691
• Central to G2/M transition: phosphorylates CCNB1, CDC25C, FOXM1, and others to promote CDK1 activation. Related references: PMID:11202906 PMID:12447691 PMID:12524548
• Essential for centrosome function and spindle assembly by phosphorylating KIZ, NEDD1, and NINL. Related references: PMID:16980960 PMID:19509060 PMID:12852856
• Critical for mitotic exit and cytokinesis via phosphorylation of CEP55, ECT2, KIF20A, PRC1, RACGAP1, and cleavage furrow formation. Related references: PMID:12939256 PMID:16247472 PMID:17351640 PMID:19468300 PMID:19468302
• Regulates kinetochore function and chromatid cohesion via phosphorylation of BUB1B, FBXO5/EMI1, STAG2/SA2; required for BUB1B kinetochore localization. Related references: PMID:15148369 PMID:15469984 PMID:17376779 PMID:17617734
• Negatively regulates p53 by phosphorylating TOPORS, leading to p53 degradation. Related references: PMID:19473992
• Localizes to nucleus, centrosome, kinetochores, spindle, and midbody; primarily expressed in placenta and colon.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole cell lysates from human, mouse, rat, or monkey cells and validate with a known positive control.
• The immunogen (aa 272-495) corresponds to the C-terminal region containing the POLO box domains; expect specific recognition of PLK1.
• This antibody is suitable for ELISA; optimize coating and detection conditions for best results.
• Upon receipt, store the antibody as recommended (ship on ice bag) and aliquot to avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering high-quality monoclonal antibodies, validated antibody pairs, and bulk ancillary reagents. This anti-PLK1 monoclonal antibody supports research in cell cycle regulation and oncology. We also specialize in sourcing rare target raw materials to accelerate your diagnostic development from concept to clinic.