Anti-PKC delta Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q05655

Core Product Specifications and Parameters

Biological Background: PKC delta Function and Localization

• PKC delta, encoded by the PRKCD gene, is a member of the protein kinase C family and functions as a calcium-independent, diacylglycerol-activated serine/threonine kinase.
• It plays dual roles in apoptosis: pro-apoptotic upon DNA damage by activating p53/TP53 via BCLAF1 or CHUK/IKKA, and anti-apoptotic during cytokine receptor-initiated cell death; also mediates TRAIL-induced survival in glioma cells (PubMed:21406692, PubMed:21810427, PubMed:15774464). Related references: PMID:21406692, PMID:21810427, PMID:15774464
• Involved in cell cycle regulation, arresting G1/S via CDKN1A/p21 and inhibiting CCNA2, and phosphorylating CDK1 for G2/M checkpoint after UV damage; also promotes cancer cell survival by inducing cyclin D1 and activating NF-kappa-B, AKT1, and MAPK pathways.
• Regulates innate immunity: required for NADPH oxidase activity and superoxide production in neutrophils and eosinophils by phosphorylating NCF1/p47-phox, and mediates antifungal immunity via CARD9 phosphorylation (PubMed:19801500, PubMed:11748588). Related references: PMID:19801500, PMID:11748588
• Modulates platelet function: negative regulator of filopodia formation by interacting with VASP, and differentially regulates dense granule secretion downstream of PAR and CD36 receptors (PubMed:16940418, PubMed:19587372). Related references: PMID:16940418, PMID:19587372
• Localizes to cytoplasm, nucleus, perinuclear region, cell membrane, mitochondrion, and endomembrane system, reflecting its diverse signaling roles.
• Contains C2-like and catalytic kinase domains with zinc-finger motifs, and is regulated by phosphorylation; multiple alternatively spliced isoforms exist.

Experimental Guidance and Technical Tips

• Immunogen design: This antibody was raised against a recombinant protein corresponding to amino acids 547-653 near the C-terminus of human PKC delta. Consider verifying epitope specificity for your experimental context.
• Western blotting (WB): Predicted molecular weight is 78 kDa. Use appropriate protein loading controls and consider phosphatase inhibitors to preserve phosphorylation status.
• Immunohistochemistry (IHC-P): Antigen retrieval may be necessary for formalin-fixed paraffin-embedded tissues. Optimize retrieval buffer and detection system for your samples.
• Immunofluorescence (IF/ICC): Permeabilization and fixation conditions should be optimized. Nuclear and cytoplasmic localization may be observed.
• ELISA: Suitable for direct or capture ELISA; validate with appropriate standards and controls.
• Cross-reactivity: Reported to react with human, mouse, and rat. Confirm reactivity in your species and tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to supply high-quality IVD raw materials, including validated monoclonal antibodies like this anti-PKC delta antibody for apoptosis and cell signaling research. From antibody pairs and bulk ancillary reagents to rare target sourcing, we streamline procurement so you can accelerate kit development and biomarker validation. Contact us to explore customized sourcing solutions for your PKC delta-related projects.