Anti-DKC1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O60832

Core Product Specifications and Parameters

Biological Background: DKC1 Function and Localization

• Catalytic subunit of H/ACA small nucleolar ribonucleoprotein (snoRNP) complex that catalyzes pseudouridylation of rRNA, critical for ribosome biogenesis (PubMed:25219674, PubMed:32554502). Related references: PMID:25219674 PMID:32554502
• Required for telomere maintenance by correctly processing and trafficking TERC, the RNA component of telomerase (PubMed:19179534). Related references: PMID:19179534
• Promotes cell-to-cell adhesion, increases proliferation rate, and induces cytokeratin hyper-expression.
• Ubiquitously expressed in human tissues.
• Localizes to the nucleolus, Cajal bodies, and cytoplasm.
• Post-translational modifications include acetylation and phosphorylation; mutations linked to dyskeratosis congenita, a telomere biology disorder.
• Functions as a pseudouridine synthase (isomerase) with RNA-binding activity.

Experimental Guidance and Technical Tips

• For Western blot (WB), a band at approximately 58 kDa is expected. Validate using positive control lysates from HeLa, Jurkat, or other human, mouse, or rat cell lines.
• For immunohistochemistry (IHC-P), test on formalin-fixed paraffin-embedded tissue sections with appropriate antigen retrieval methods; nuclear/nucleolar staining should be observed.
• For ELISA, as a monoclonal antibody, it can be used as either capture or detection antibody; verify pairing compatibility with other anti-DKC1 antibodies in your system.
• Since the immunogen is a peptide within amino acids 300-400, the antibody likely recognizes total DKC1; consider blocking peptides for specificity control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs and high-quality monoclonal antibodies like this anti-DKC1 product. Whether you need bulk ancillary reagents or rare target raw-material sourcing for telomere biology or ribosome biogenesis studies, our one-stop supply platform bridges the gap from concept to clinic.