Rabbit anti-Human PD-L1/CD274 mAb - Q9NZQ7

Core Product Specifications and Parameters

Biological Background: PD-L1/CD274 Function and Localization

• Protein Identity and Family: PD-L1/CD274, also known as B7 homolog 1 (B7-H1), B7H1, PDL1, and PDCD1LG1, is a member of the B7 family of immune regulatory ligands.
• Immune Tolerance: PD-L1 binds the inhibitory receptor PD-1 on T cells, raising the activation threshold and limiting effector responses, which is critical for maintaining self-tolerance. Related references: PMID:11015443 PMID:28813410 PMID:28813417
• Tumor Immune Evasion: Tumor cells exploit PD-L1 to suppress anti-tumor immunity by inhibiting CTL function. Blocking PD-1/PD-L1 reverses T-cell exhaustion and enhances anti-tumor responses. Related references: PMID:28813410 PMID:28813417
• Subcellular Distribution: PD-L1 localizes to the plasma membrane, early and recycling endosomes, the nucleus, and can be secreted.
• Tissue and Cellular Expression: Highly expressed in heart, skeletal muscle, placenta, and lung; lower levels in immune organs. Induced on activated T cells, B cells, dendritic cells, and monocytes.
• Additional Functions: PD-L1 may costimulate IL-10-producing T-cell subsets via an unknown receptor (PubMed:10581077), and under hypoxia, it acts as a transcriptional coactivator to promote pyroptosis (PubMed:32929201). Related references: PMID:10581077 PMID:32929201

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 1-290), which is suitable for targeting surface-expressed PD-L1 in flow cytometry.
• Ensure proper sample preparation and viability for flow cytometry to maximize detection sensitivity.
• Consider titrating the antibody for optimal staining index in your specific cell system.
• Validate the antibody in the relevant human sample types where PD-L1 expression is expected (e.g., activated PBMCs, tumor cell lines).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to IVD-grade antibodies and raw materials. This PD-L1 monoclonal antibody, along with our portfolio of immune checkpoint reagents, supports immuno-oncology assay development. Our supply services include validated antibody pairs, optimized monoclonals, bulk reagents, and custom sourcing for rare targets.