Anti-PDHA1 Monoclonal Antibody for WB - P08559

Core Product Specifications and Parameters

Biological Background: PDHA1 Function and Localization

• PDHA1 is the alpha subunit of the pyruvate dehydrogenase E1 component, which together with PDHB (beta subunit) forms a heterotetrameric E1 enzyme within the pyruvate dehydrogenase (PDH) complex. Related references: PMID:17474719 PMID:19081061

• The PDH complex catalyzes the irreversible conversion of pyruvate to acetyl-CoA and CO2, thereby linking cytoplasmic glycolysis to the mitochondrial tricarboxylic acid (TCA) cycle. Related references: PMID:19081061 PMID:7782287

• Within the E1 subunit, PDHA1 performs the thiamine pyrophosphate (TPP)-dependent decarboxylation of pyruvate, followed by reductive acetylation of a lipoyl group on the E2 component, transferring the acetyl moiety for further metabolism. Related references: PMID:17474719 PMID:19081061 PMID:7782287

• The protein localizes to the mitochondrial matrix, consistent with its role in aerobic energy production.

• PDHA1 is ubiquitously expressed across human tissues, reflecting its essential housekeeping function in glucose metabolism.

• Post-translational modifications include phosphorylation and acetylation, which regulate PDH complex activity; mutations in the gene are associated with primary mitochondrial diseases such as Leigh syndrome.

• The protein is also known as PDHE1-A type I and alternative splicing generates multiple transcript variants, though the somatic form described here is the predominant isoform.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 248–348 of human PDHA1; this region is conserved in mouse, supporting the observed cross-reactivity.
• For Western blot, expect a band around 43 kDa under appropriate reducing conditions; always validate the antibody in the relevant experimental system.
• In ELISA applications, consider pairing with a suitable detection reagent; assay conditions should be optimized by the end user.
• Given the mitochondrial localization of PDHA1, ensure proper sample preparation, such as gentle lysis and possibly mitochondrial enrichment, to maximize detection.

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CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of validated IVD raw materials, including monoclonal antibodies like this anti-PDHA1 clone. Beyond individual reagents, we offer one-stop access to optimized antibody pairs, bulk ancillary reagents, and procurement of rare targets—enabling efficient development of assays focused on metabolic disorders and mitochondrial biology. Let us bridge your workflow from concept to clinic with reliable, high-quality raw materials.