Anti-PDHB Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P11177

Core Product Specifications and Parameters

Biological Background: PDHB Function and Localization

• PDHB (Pyruvate dehydrogenase E1 component subunit beta) is a mitochondrial protein that, together with PDHA1, forms the heterotetrameric E1 subunit of the pyruvate dehydrogenase (PDH) complex.
• The PDH complex catalyzes the irreversible conversion of pyruvate into acetyl-CoA and CO₂, serving as a critical link between cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle.
• Within the E1 subunit, PDHB contributes to the thiamine pyrophosphate (TPP)-dependent decarboxylation of pyruvate and the reductive acetylation of lipoyl groups on the E2 component (PubMed:17474719, PubMed:19081061). Related references: PMID:17474719 PMID:19081061
• PDHB is localized to the mitochondrial matrix.
• It undergoes post-translational modifications including acetylation and phosphorylation, which regulate PDH complex activity.
• UniProt keywords associated with PDHB include disease variant, metal-binding (potassium), and alternative splicing, highlighting its clinical relevance and potential isoform diversity.
• The protein plays a central role in glucose metabolism and is classified as an oxidoreductase.

Experimental Guidance and Technical Tips

• For Western blotting, consider using a standard blocking buffer (e.g., 5% non-fat milk in TBST) and validate the detected band around 39 kDa in relevant cell or tissue lysates.
• In immunohistochemistry (IHC-P), we recommend testing heat-induced epitope retrieval in citrate buffer (pH 6.0) for optimal signal; confirm staining in tissue sections known to express PDHB.
• When performing ELISA, the antibody can be used for target detection; titrate the antibody to determine the optimal working concentration for your specific assay format.
• As the antibody cross-reacts with mouse and rat, it is suitable for studies using rodent models; however, always validate in the relevant sample system before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for IVD raw material sourcing, offering comprehensive solutions from concept to clinic. This anti-PDHB rabbit monoclonal antibody supports research into metabolic disorders and mitochondrial biology. We provide a broad array of validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your diagnostic development and assay workflows.