Anti-PDK2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q15119

Core Product Specifications and Parameters

Biological Background: PDK2 Function and Localization

• PDK2 (pyruvate dehydrogenase kinase isoform 2) is a mitochondrial kinase encoded by the PDK2 gene. Multiple alternatively spliced isoforms have been reported.
• It phosphorylates and inactivates the pyruvate dehydrogenase complex (subunits PDHA1 and PDHA2), thereby regulating glucose and fatty acid metabolism, controlling the tricarboxylic acid cycle and aerobic respiration.
• PDK2 mediates cellular responses to insulin and plays a key role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability.
• By regulating pyruvate dehydrogenase activity, PDK2 helps maintain normal blood pH and prevents the accumulation of ketone bodies under starvation conditions.
• It is involved in the regulation of cell proliferation, resistance to apoptosis induced by oxidative stress, and p53/TP53-mediated apoptosis.
• Subcellular localization: predominantly in the mitochondrial matrix.
• Tissue expression: highest in heart and skeletal muscle, intermediate in brain, kidney, pancreas and liver, and low in placenta and lung.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 308-407) of human PDK2, a segment that may be conserved across species, consistent with the observed cross-reactivity with mouse and rat.
• In Western blotting, expect a major band around 46 kDa and a possible additional band at 39 kDa, reflecting different isoforms or processing variants; validate with appropriate positive and negative controls.
• For immunohistochemistry (IHC-P), consider standard antigen retrieval procedures and include tissue-specific controls, especially heart and skeletal muscle where PDK2 is highly expressed.
• For ELISA applications, the antibody can be tested as a capture or detection reagent; optimal working concentrations should be determined experimentally in the relevant matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop platform for IVD raw material sourcing, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Our anti-PDK2 antibody supports research in metabolism, diabetes, and cell signaling. For rare targets or customized procurement needs, we leverage extensive global supply networks to accelerate your diagnostic development from concept to clinic.