Anti-PDK1/PDHK1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q15118

Core Product Specifications and Parameters

Biological Background: PDK1/PDHK1 Function and Localization

• PDK1 is a mitochondrial kinase that phosphorylates and inactivates the pyruvate dehydrogenase complex (PDC) by targeting subunits PDHA1 and PDHA2, thereby regulating glucose and fatty acid metabolism. Related references: PMID:7499431 PMID:18541534 PMID:22195962
• This phosphorylation inhibits pyruvate dehydrogenase activity, reducing acetyl-CoA production from pyruvate and down-regulating aerobic respiration and the TCA cycle. Related references: PMID:18541534 PMID:22195962 PMID:26942675
• PDK1 plays a critical role in the cellular response to hypoxia and promotes cell proliferation under low oxygen conditions. Related references: PMID:18541534 PMID:22195962 PMID:26942675
• PDK1 is predominantly expressed in the heart, with lower expression in liver, skeletal muscle, and pancreas.
• Located in the mitochondrial matrix, where it exerts its regulatory functions.
• The protein is subject to alternative splicing and phosphorylation, and belongs to the PDK/BCKDK protein kinase family.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 337-436) is located in the C-terminal region, which may contribute to cross-reactivity across human, mouse, and rat.
• For Western blot, a band around 49 kDa is expected; validate with appropriate positive controls.
• For IHC-P, consider antigen retrieval methods and validate in relevant tissue types such as heart or skeletal muscle.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD antibodies and raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For researchers studying metabolic disorders, hypoxia, or mitochondrial diseases, CamelBio can source rare-target raw materials and provide technical support for assay development. Contact us for tailored solutions from concept to clinic.